P8287
o-Phenylenediamine dihydrochloride
chromogenic, tablet
Synonym(s):
OPD, 1,2-Phenylenediamine tablet, OPD Tablet
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About This Item
Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
MDL number:
Beilstein/REAXYS Number:
3912045
Product Name
o-Phenylenediamine dihydrochloride, tablet, 10 mg substrate per tablet
Quality Level
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
form
tablet
mp
258 °C (dec.) (lit.)
storage temp.
2-8°C
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Application
o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibody in enzyme-linked immunosorbent assay (ELISA).
General description
o-Phenylenediamine (o-PD) is a colorless and nonfluorescent molecule.
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
Preparation Note
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
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Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
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