217669 Anti-CD28 Mouse mAb (ANC28.1/5D10)

217669
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
217669-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes CD28 on human T cells and BHK cells transfected with human CD28.
      Catalogue Number217669
      Brand Family Calbiochem®
      References
      ReferencesKariv, I., et al. 1996. J. Immunol. 157, 29.
      61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
      Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Preservative500 µg/ml gentamycin sulfate
      Quality LevelMQ100
      Applications
      Key Applications T Cell Co-stimulation
      Flow Cytometry
      Frozen Sections
      Immunoprecipitation
      Application NotesFlow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      T cell Co-stimulation (see comments)
      Application CommentsMonomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman CD28 Ig fusion protein and HPB-MLT human tumor cells
      ImmunogenHuman
      CloneANC28.1/5D10
      HostMouse
      Purity>95% immunoglobulin by SDS-PAGE
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CD28 Mouse mAb (ANC28.1/5D10) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD28 Mouse mAb (ANC28.1/5D10) Certificates of Analysis

      TitleLot Number
      217669

      References

      Reference overview
      Kariv, I., et al. 1996. J. Immunol. 157, 29.
      61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
      Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.

      Citations

      Title
    • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      ApplicationFlow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      T cell Co-stimulation (see comments)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes the CD28 protein.
      BackgroundHuman CD28 is an important co-stimulatory molecule found on all CD4+ T cells and ~50% of CD8+ T cells. T cell activities attributed to CD28 include prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNA, and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, known as CD80 (B7-1) and CD86 (B7-2), are monomeric transmembrane glycoproteins.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman CD28 Ig fusion protein and HPB-MLT human tumor cells
      CloneANC28.1/5D10
      IsotypeIgG₁
      Specieshuman
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity>95% immunoglobulin by SDS-PAGE
      Preservative500 µg/ml gentamycin sulfate
      CommentsMonomeric and cross-linked antibody will stimulate expression of IL-2 from CD28+ cells. FACS was performed on 5x105 cultured HPB-MLT cells using anti-CD28 antibody at 5 µg/ml and FITC-conjugated goat anti-mouse IgG. This antibody is also reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesKariv, I., et al. 1996. J. Immunol. 157, 29.
      61st Forum in Immunology. 1995. Res. Immunol. 146, 127.
      Jenkins, M.K., and Johnson, J.G. 1993. Curr. Opin. Immunol. 5, 361.
      Citation
    • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.