217594 Anti-CD44 Rat mAb (A020)

217594
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, H, M, Porcine, RbRMonoclonal Antibody

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
217594-100UL
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      Plastic ampoule 100 ul
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      Description
      OverviewRecognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.
      Catalogue Number217594
      Brand Family Calbiochem®
      References
      ReferencesIida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
      Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesNeutralization Studies Bourguignon, L.Y.W., et al. 2007. J. Biol. Chem. 282, 1265. Bourguignon, L.Y.W., et al. 2006. J. Biol. Chem. 281 14026.
      Key Applications Immunofluorescence
      Immunohistochemistry
      Immunoprecipitation
      Radioimmunoassay
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Immunoblotting (Western Blotting)
      Application NotesELISA (1:2000)
      Flow Cytometry (1:1000)
      Immunoblotting (1:1000)
      Immunofluorescence (1:200)
      Immunohistochemistry (1:250)
      Immunoprecipitation (1:200)
      Neutralization Studies (see comments and application references)
      Radioimmunoassay (1:5000)
      Application CommentsThe antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified human lymphocyte CD44
      ImmunogenHuman
      CloneA020
      HostRat
      IsotypeIgG2b
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Mouse
      • Porcine
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-CD44 Rat mAb (A020) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD44 Rat mAb (A020) Certificates of Analysis

      TitleLot Number
      217594

      References

      Reference overview
      Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
      Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      ApplicationELISA (1:2000)
      Flow Cytometry (1:1000)
      Immunoblotting (1:1000)
      Immunofluorescence (1:200)
      Immunohistochemistry (1:250)
      Immunoprecipitation (1:200)
      Neutralization Studies (see comments and application references)
      Radioimmunoassay (1:5000)
      DescriptionRat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
      HostRat
      Immunogen speciesHuman
      Immunogenpurified human lymphocyte CD44
      CloneA020
      IsotypeIgG2b
      Speciesbovine, canine, human, mouse, porcine, rabbit
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesIida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol. 162, 121.
      Bourguignon, L.Y.W., et al. 1993. J. Immunol. 151, 6634.
      Application referencesNeutralization Studies Bourguignon, L.Y.W., et al. 2007. J. Biol. Chem. 282, 1265. Bourguignon, L.Y.W., et al. 2006. J. Biol. Chem. 281 14026.