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MABE1784-100UG Anti-NCoA-2 Antibody, clone 3Ti-3C11

This mouse monoclonal Anti-NCoA-2, clone 3Ti-3C11 , Cat. No. MABE1784 is tested for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), and ELISA, for the detection of NCoA-2.

MABE1784-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1784-100UG
      DescriptionAnti-NCoA-2 Antibody, clone 3Ti-3C11
      Alternate Names
      • NCoA-2
      • Class E basic helix-loop-helix protein 75 (bHLHe75)
      • Transcriptional intermediary factor 2 (hTIF2)
      • SRC2
      Background InformationNuclear receptor coactivator 2 (UniProt: Q15596; also known as NCoA-2, Transcriptional intermediary factor 2, hTIF2, Class E basic helix-loop-helix protein 75 (bHLHe75)) is encoded by the NCOA2 (also known as BHLHE75, SRC2, TIF2) gene (Gene ID: 10499) in human. Nuclear receptor coactivator 2 (NCoA-2) functions as a transcriptional coactivator in various cellular processes. It belongs to the p160/steroid receptor coactivator (SRC) family of proteins. NCoA-2 plays a crucial role in the regulation of gene expression by interacting with nuclear receptors and other transcription factors. It possesses multiple functional domains, including a nuclear receptor interaction domain (NRID), a histone acetyltransferase (HAT) domain, and several transcription activation domains (ADs). NCoA-2 acts as a bridge between transcription factors and the transcriptional machinery, facilitating the recruitment of coactivators and chromatin remodeling complexes to target gene promoters. This coactivator function enables NCoA-2 to modulate diverse biological processes such as development, metabolism, cell proliferation, and differentiation. Dysregulation of NCoA-2 has been implicated in various diseases, including cancer, where it can promote tumor growth and progression.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-NCoA-2, clone 3Ti-3C11 , Cat. No. MABE1784 is tested for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), and ELISA, for the detection of NCoA-2.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • ELISA
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NCoA-2 in Chromatin Immunoprecipitation application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508; Ganti, K.P., et al. (2017). Proc Natl Acad Sci USA. 114(6):E951-E960).

      Western Blotting Analysis: A representative lot detected NCoA-2 in Western Blotting application. (Rambaud, J., et al. (2009). J Biol Chem. 284(21):14147-56; Duteil, D., et al. (2010). Cell Metab. 12(5):496-508).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged, recombinant, human NCoA-2 protein fragment (aa 624-1287).
      EpitopeN-terminal half
      Clone3Ti-3C11
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3Ti-3C11 mouse monoclonal antibody that detects NCoA-2. It targets an epitope within 246 amino acids from the internal part of the protein.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Horse, Feline based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCOA2
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~85 kDa observed. 159.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with His-tagged, recombinant fragment of Human NCoA-2.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, recombinant fragment (aa 624-1287) of Human NCoA-2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1784-100UG 04065271174986