218701 Casein Kinase II, Human, Recombinant, E. coli

218701
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218701-10KU
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      Description
      OverviewRecombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits (M.W. 44 kDa) and regulatory β-subunits (M.W. 26 kDa) in an α2β2-tetramer. This material corresponds to one of several forms of native CKII. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies.
      Note: 1 KU = 1000 units.
      Catalogue Number218701
      Brand Family Calbiochem®
      SynonymsrhCKII
      References
      ReferencesYamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
      Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
      Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.
      Product Information
      Activity≥500,000 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.
      FormLiquid
      FormulationIn 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON® X-100 Detergent, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Casein Kinase II, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Casein Kinase II, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      218701

      References

      Reference overview
      Yamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
      Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
      Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision18-November-2008 JSW
      SynonymsrhCKII
      DescriptionRecombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits (M.W. 44 kDa) and regulatory β-subunits (M.W. 26 kDa) in an α2β2- tetramer. This material is one of several forms of native CK-II. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies. The following substrates have been characterized by CK-II both in vitro and in vivo: nucleolin, RNA polymerase, p53, and SV 40 large T antigen.
      FormLiquid
      FormulationIn 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON® X-100 Detergent, pH 7.5.
      Recommended reaction conditions20 mM Tris-HCl, pH 7.5, 50 mM KCl, 10 mM MgCl2, and 200 μM ATP (200 μCi/μmol). GTP can be used as a phosphoryl donor in place of ATP. Incubate at 30°C. Note: optimal incubation times and enzyme concentrations must be determined empirically for each substrate.
      Activity≥500,000 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesYamaguchi, Y., et al. 1998. Nucleic Acids Res. 26, 3854.
      Chester, N., and Marshak, D.R. 1993. Anal. Biochem. 209, 284.
      Russo, G.L., et al. 1992. J. Biol. Chem. 267, 20317.