218807 Caspase-9, Human, Recombinant, E. coli

218807
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218807-100U
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      Description
      OverviewRecombinant, human caspase-9 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.
      Catalogue Number218807
      Brand Family Calbiochem®
      SynonymsICE-LAP6, Mch6
      References
      ReferencesQin, H., et al. 1999. Nature 399, 549.
      Li, P., et al. 1997. Cell 91, 479.
      Salvesen, G.S., et al. 1997. Cell 91, 443.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=218805">Cat. No. 218805</a>) per h at 37°C, pH 7.2.
      FormLyophilized
      FormulationLyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥70% by SDS-PAGE
      Specific Activity≥400 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Caspase-9, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Caspase-9, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      218807

      References

      Reference overview
      Qin, H., et al. 1999. Nature 399, 549.
      Li, P., et al. 1997. Cell 91, 479.
      Salvesen, G.S., et al. 1997. Cell 91, 443.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-June-2008 RFH
      SynonymsICE-LAP6, Mch6
      DescriptionRecombinant, human caspase-9 fused at the N- and C- termini to a His•Tag® sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.
      FormLyophilized
      FormulationLyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄
      Recommended reaction conditions50 mM HEPES, 50 mM NaCl, 10 mM DTT, 10 mM EDTA, 5% glycerol, 0.1% CHAPS, pH 7.2. Recommended use: 1 unit per assay.
      Purity≥70% by SDS-PAGE
      Specific activity≥400 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.
      SolubilityPBS containing 15% glycerol (1 U/µl)
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.
      Toxicity Standard Handling
      ReferencesQin, H., et al. 1999. Nature 399, 549.
      Li, P., et al. 1997. Cell 91, 479.
      Salvesen, G.S., et al. 1997. Cell 91, 443.