218809 Caspase Substrate Set IV, Fluorogenic - Calbiochem

218809
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      Description
      OverviewReady-to-use substrates for assaying the activity of various members of the caspase-family proteases. Contains 125 µl (1 mM) each of Caspase-1 Substrate VI, Ac-YVAD-AFC (Cat. No. 688224); Caspase-2 Substrate I, Ac-VDVAD-AFC (Cat. No. 218740); Caspase-3 Substrate IV, Ac-DEVD-AFC (Cat. No. 264150); Caspase-5 Substrate II, Ac-WEHD-AFC (Cat. No. 218754); Caspase-6 Substrate III, Ac-VEID-AFC (Cat. No. 218763); Caspase-8 Substrate I, Z-IETD-AFC (Granzyme B Substrate II, Cat. No. 368059); and Caspase-9 Substrate I, Ac-LEHD-AFC (Cat. No. 218765). Supplied with a data sheet.
      Catalogue Number218809
      Brand Family Calbiochem®
      Application Data
      References
      ReferencesHumke, E.W., et al. 1998. J. Biol. Chem. 273, 15702.
      Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123.
      Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
      Nicholson, D.W., et al. 1995. Nature 376, 37.
      Thornberry, N.A., et al. 1992. Nature 356, 768.
      Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.
      Product Information
      FormLiquid
      FormulationEach substrate supplied at 1 mM in DMSO.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/38

      Irritating to eyes and skin.
      S PhraseS: 26

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Caspase Substrate Set IV, Fluorogenic - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Caspase Substrate Set IV, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      218809

      References

      Reference overview
      Humke, E.W., et al. 1998. J. Biol. Chem. 273, 15702.
      Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123.
      Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
      Nicholson, D.W., et al. 1995. Nature 376, 37.
      Thornberry, N.A., et al. 1992. Nature 356, 768.
      Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-September-2014 JSW
      Application Data
      DescriptionApoptosis is a normal process in development and morphogenesis. However, improperly regulated apoptosis can contribute to several pathological conditions including cancer, Alzheimer's disease, spinal muscular atrophy, ischemic cardiac damage, and autoimmune syndromes. Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. Receptor-mediated apoptosis involves the catalysis of preexisting intracellular caspase proenzymes into activated caspases (cysteine-containing aspartate-specific proteases). A distinctive feature of caspases is the requirement of an aspartic acid residue in the substrate P1 position. The P4 residue is important in substrate recognition and specificity. For example, caspase-1 prefers a hydrophobic tyrosine residue in the P4 position, but caspase-3 prefers an anionic aspartic residue. Caspase activity can be assayed using a fluorophore (AFC). Liberation of the fluorophore from the substrate is monitored to determine caspase activity. Supplied with a data sheet.

      This convenient set of caspase substrates contains the following components, each at a concentration of 1 mM in DMSO:
      FormLiquid
      FormulationEach substrate supplied at 1 mM in DMSO.
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesHumke, E.W., et al. 1998. J. Biol. Chem. 273, 15702.
      Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Takahashi, A., et al. 1997. Exp. Cell Res. 231, 123.
      Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.
      Nicholson, D.W., et al. 1995. Nature 376, 37.
      Thornberry, N.A., et al. 1992. Nature 356, 768.
      Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.