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Protein Visualization
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
DNA / Protein Electrophoresis and Troubleshooting Tables
DNA / Protein Electrophoresis and Troubleshooting Tables
Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System
Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.
Preparing for IEF
This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.
Reproducibility with Biological Buffers
Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.
Formamide Molecular Biology Reagent
Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.
Paper-Bridge Loading
This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products
Applying Equilibrated Immobiline® DryStrip Gels
This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.
Detection of GST-tagged Proteins with Western Blot
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
Proteomic analysis of membrane proteins
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.
Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions
Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.
How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System
Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.
Winning Westerns Technique Talk: Perfecting Western Blotting
In this webinar, Alli Lynch, Ph.D. reviews the basics of Western blotting, identifies common troubleshooting issues, and provides tips for Western blotting success.
Precast Agarose Gels for RNA Electrophoresis
Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.
Auto2D® Automated 2-D Gel Electrophoresis Device Protocol
The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.
ReadyBlue Protein Gel Staining Protocols
ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.
Northern & Southern Blot Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Two-Dimensional Separation of Protein Samples
This page describes two-dimensional separation of protein samples with Cytiva products.
Sample Prep & Gel Electrophoresis Troubleshooting
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Sample Prep & Gel Electrophoresis Troubleshooting
Identify causes and remedies for SDS-PAGE sample preparation challenges and optimize electrophoresis conditions.
Protein Staining Reagents Selection Guide
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Protein Staining Reagents Selection Guide
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Troubleshooting 2-D DIGE Results
Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.
Troubleshooting 2-D DIGE Results
Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.
Auto2D® Gel Electrophoresis Device
Auto2D® automates difficult 2D electrophoresis methods for protein localization with high reproducibility in under two hours.
Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
Water for Protein Electrophoresis & Western Blotting
Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.
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