Anti-Bmi-1 Antibody, clone F6, Alexa Fluor^™ 647

40-44 kDa observed

Polycomb complex protein BMI-1 (UniProt P25916; also known as B lymphoma Mo-MLV insertion region 1, Polycomb group RING finger protein 4) is encoded by the Bmi1 (also known as AW546694, Bmi-1, Pcgf4) gene (Gene ID 12151) in murine species. Bmi1 is a member of the polycomb group (PcG) family of evolutionarily conserved gene silencers involved in cell fate decisions and body pattern during development. Bmi1 is an important regulator of self-renewal of hematopoietic and neural stem cells, as well as cancer stem cells (CSCs). Bmi1 is a main constituent of the polycomb repressive complex 1 (PRC1) and pariticipates in PRC-mediated gene silencing by promoting monoubiquitination of H2A. Bmi1 overexpression is reported in many different human cancers and high level of Bmi1 is considered as a poor prognostic marker. Bmi1 targets genes involved in transforming growth factor-β/bone morphogenetic protein (TGF-beta/BMP), endoplasmic reticulum (ER) stress, and WNT pathways. Bmi itself is transcriptionally regulated by c-Myc and E2F1 transcription factors.

Epitope: a.a. 1-202

Recombinant Bmi-1 protein corresponding to residues 1-202 of mouse Bmi-1.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in HepG2 cells.
The unconjugated version (Cat. No. 05-637) is also shown to be suitable for Western blotting, Immunocytochemistry, and Immunohistochemistry applications.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-Bmi-1 Antibody, clone F6, Alexa Fluor^™ 647 is validated for use in Immunocytochemistry for the detection of Bmi-1.

Protein G Purified

Purified mouse conjugate antibody in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bmi-1 in HeLa cells.

Concentration: Please refer to lot specific datasheet.

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