1.25058
Fractogel® EMD DEAE (M)
MiniChrom Fractogel® DEAE (M), volume 1 mL, prepacked column
Synonym(s):
MiniChrom Column Fractogel® DEAE (M), 1 ml
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About This Item
UNSPSC Code:
41115711
ligand
diethylaminoethyl
material
polypropylene housing
product line
Fractogel®
form
prepacked column
parameter
≤8 bar pressure, ~170 cm/hr max. flow rate
column I.D. × L
8 mm × 20 mm
volume
1 mL
matrix active group
Methacrylate
mean particle size
40-90 μm
capacity
~100 mg, BSA binding capacity
application(s)
gene therapy
mAb
vaccine development
separation technique
anion exchange
storage temp.
15-25°C
General description
8 x 20mm
Features and Benefits
MiniChrom Column Fractogel® DEAE (M), 1 ml enables:
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- Excellent binding to large viruses and plasmid DNA
- Homogenous binding with high selectivity and purity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the degree of the ionization of the functional groups of the exchanger
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
Analysis Note
Appearance: passes test
Legal Information
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
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