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Merck

1.06177

TBE buffer

10 x pH 8.3 tris-borate-EDTA buffer

Synonym(s):

10X TBE running buffer, Tris-Borate EDTA buffer

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About This Item

NACRES:
NA.25
UNSPSC Code:
41105319

Key Documents

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Product Name

TBE buffer, 10 x pH 8.3 tris-borate-EDTA buffer

form

liquid

pH

8.2-8.4 (20 °C in H2O)

density

1.06 g/cm3 at 20 °C

storage temp.

15-25°C

Related Categories

Analysis Note

Appearance (colour): almost colourless
Appearance (description): almost clear
pH-value: 8.2 - 8.4

General description

Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO- species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg2+ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments. It also protects nucleic acids from enzymatic degradation.

pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - Repr. 1B - Skin Sens. 1

Storage Class

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric
Optical properties of buffers and cell culture media for optofluidic and sensing applications
Hoang VT, et al.
Applied Sciences, 9(6), 1145-1145 (2019)

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