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14-756M ACK1 Protein, active, 250 µg

Active, N-terminal GST-tagged recombinant, human ACK1, residues 1-389. For use in Kinase Assays.

ACK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-756M
250 µg  Also available in 10 μg size (cat#14-756) and in bulk (cat# 14-756-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-756M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionACK1 Protein, active, 250 µg
OverviewN-terminal GST-tagged recombinant, human ACK1, residues 1-389.
Alternate Names
  • Activated CDC42 kinase 1
  • ACK-1
  • Tyrosine kinase non-receptor protein 2
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal GST-tagged recombinant, human ACK1, residues 1-389. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
Gene Symbol
  • TNK2
  • FLJ44758
  • ACK1
  • p21cdc42Hs
  • ACK
  • ACK-1
  • FLJ45547
Protein TargetACK1
Purification MethodGlutathione-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q07912 # Downstream effector of CDC42 which mediates CDC42- dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development.
COFACTOR: Magnesium.
SIZE: 1036 amino acids; 114327 Da
SUBUNIT: Interacts with CDC42. Interacts with activated CSPG4.
SUBCELLULAR LOCATION: Cell membrane (By similarity).
SIMILARITY: SwissProt: Q07912 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 1 CRIB domain. & Contains 1 protein kinase domain. & Contains 1 SH3 domain.
Molecular Weight71.5 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions-70°C from date of shipment.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-756) and in bulk (cat# 14-756-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-756M 04053252478895