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MABN757 Anti-Beta-glucosidase 2 (GBA2) Antibody, clone 4A12

Anti-Beta-glucosidase 2 (GBA2) Antibody, clone 4A12, Cat. No. MABN757, is a highly specific rat monoclonal antibody that targets beta-glucosidase 2 (GBA2) and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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Research Category: Neuroscience Gene Symbol: Gba2, Kiaa1605 UniProt Number: Q69ZF3
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MABN757
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIH(P), ICC, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN757
DescriptionAnti-Beta-glucosidase 2 (GBA2) Antibody, clone 4A12
Alternate Names
  • Non-lysosomal glucosylceramidase
  • Beta-glucocerebrosidase 2
  • Beta-glucosidase 2
  • Glucosylceramidase 2
  • NLGase
Background InformationNon-lysosomal glucosylceramidase (EC 3.2.1.45; UniProt Q69ZF3; also known as Beta-glucocerebrosidase 2, Beta-glucosidase 2, Glucosylceramidase 2, NLGase) is encoded by the Gba2 (also known as Kiaa1605) gene (Gene ID 230101) in murine species. Glucosidases GBA1, GBA2, and GBA3 are structurally unrelated proteins with similar hydrolase activity that mediates glucosylceramide (GlcCer) degradation. GBA1 mediates GlcCer degradation in the lysosome lumen following its transport via the endosomal pathway. GBA2 is localized at the cytosolic surface of the ER and Golgi, with a predominant presence at the cis-Golgi in close proximity to the site of GlcCer synthesis and sphingomyelin conversion. GlcCer is also delivered to the ER through FAPP2-dependent transport and then flips to ER lumenal side by low specificity phospholipid flippases. GBA2 ER localization allows it to regulate GlcCer level at the cytosolic side of the ER and limit the amount of GlcCer from entering the ER lumenal side for higher order glycosphingolipid synthesis. Impaired GBA1 activity causes accumulation of GlcCer in lysosomes of tissue macrophages, causing liver and spleen enlargement and impairment of the central nervous system in patients with Gaucher disease. GBA2 activity is found downregulated in fibroblasts from a patient with type II Gaucher disease and fibroblasts from Gba1-deficient mice, suggesting a cross-talk between GBA1 and GBA2 that could affect the phenotype of Gaucher disease. GBA3 is a cytosolic Klotho-related protein whose physiological function is not yet known.
References
Product Information
FormatPurified
PresentationPurified rat IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Beta-glucosidase 2 (GBA2) Antibody, clone 4A12, Cat. No. MABN757, is a highly specific rat monoclonal antibody that targets beta-glucosidase 2 (GBA2) and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized, but not non-permeabilized, HEK293 transfectants expressing HA-tagged mouse beta-glucosidase 2 (GBA2-HA). GBA2-HA immunoreactivity was found associated with both Golgi and ER markers and greatly dependent on microtubule dynamics (Körschen, H.G., et al. (2013). J. Biol. Chem. 288(5):3381-3393).

Immunocytochemistry Analysis: A representative lot detected beta-glucosidase 2 (GBA2) in isolated embryonic mouse hippocampal neurons, but not astrocytes (Körschen, H.G., et al. (2013). J. Biol. Chem. 288(5):3381-3393).

Western Blotting Analysis: A representative lot detected HA-tagged mouse beta-glucosidase 2 exogenously expressed in HEK293 cells, as well endogenous beta-glucosidase 2 in testis tissue samples from wild-type, but not Gba2-knock mice (Körschen, H.G., et al. (2013). J. Biol. Chem. 288(5):3381-3393).
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to a sequence from the N-terminal region of mouse beta-glucosidase 2 (GBA2).
Clone4A12
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 4A12 detected the beta-glucosidase 2 (GBA2) target band in testis tissue sample from wild-type, but not Gba2-knock mice (Körschen, H.G., et al. (2013). J. Biol. Chem. 288(5):3381-3393).
IsotypeIgG1κ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Gba2
  • Kiaa1605
Purification MethodProtein G purified.
UniProt Number
Molecular Weight103.3 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in mouse brain tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-glucosidase 2 (GBA2) in mouse brain tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN757 04054839069611