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MABT217 Anti-Calreticulin Antibody, clone 16B11.1

This Anti-Calreticulin Antibody, clone 16B11.1 is validated for use in Western Blotting, IHC, Flow Cytometry for the detection of Calreticulin.

Anti-Calreticulin Antibody, clone 16B11.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CALR, CRTC UniProt Number: P27797
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MABT217
100 µL  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT217
ReplacesMABT145
DescriptionAnti-Calreticulin Antibody, clone 16B11.1
Alternate Names
  • Calreticulin
  • CRP55
  • Calregulin
  • Endoplasmic reticulum resident protein 60
  • ERp60
  • HACBP
  • grp60
Background InformationCalreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters. Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER. Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Calreticulin Antibody, clone 16B11.1 is validated for use in Western Blotting, IHC, Flow Cytometry for the detection of Calreticulin.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Flow Cytometry
Application NotesImmunohistochemistry Analysis: A 1:300-1,000 dilution from a representative lot detected Calreticulin in human colorectal cancer and human tonsil tissues.

Flow Cyometry Analysis: A 1:500 dilution from a representative lot detected Calreticulin in non serum starved HeLa cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human Calreticulin.
Clone16B11.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CALR
  • CRTC
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~48 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Calreticulin on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT217 04053252646324

Documentation

Anti-Calreticulin Antibody, clone 16B11.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-Calreticulin Antibody, clone 16B11.1 Certificates of Analysis

TitleLot Number
Anti-Calreticulin, clone 16B11.1 2487734
Anti-Calreticulin, clone 16B11.1 - 2452664 2452664
Anti-Calreticulin, clone 16B11.1 - 2464644 2464644
Anti-Calreticulin, clone 16B11.1 - 3234490 3234490
Anti-Calreticulin, clone 16B11.1 - 4101908 4101908
Anti-Calreticulin, clone 16B11.1 - Q2015737 Q2015737
Anti-Calreticulin, clone 16B11.1 -2683318 2683318
Anti-Calreticulin, clone 16B11.1 -2736482 2736482
Anti-Calreticulin, clone 16B11.1 -2740941 2740941
Anti-Calreticulin, clone 16B11.1 -2761621 2761621
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Brochure

Title
New Products: Volume 3, 2012