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AB5850 Anti-GABA B Receptor R1 Antibody

This Anti-GABA B Receptor R1 Antibody is validated for use in WB for the detection of GABA B Receptor R1.

Anti-GABA B Receptor R1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: GABBR1, dJ271M21.1.1, dJ271M21.1.2, Gb1, GABA-B-R1, GPRC3A, GABBR1-3, GABAB(1e), hGB1a, GABABR1 UniProt Number: Q9UBS5
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AB5850
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, SpdWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberAB5850
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-GABA B Receptor R1 Antibody
References
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid. Buffer = PBS containing 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-GABA B Receptor R1 Antibody is validated for use in WB for the detection of GABA B Receptor R1.
Key Applications
  • Western Blotting
Application NotesImmunoblotting: 1:500-1:1,000. The antibody should detect a ~106kDa band under standard western conditions.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogenamino acid 33-54 of the rat GABA(B)R1 protein (PHLPRPHPRVPPHPSSERRAVY)
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityGABAB Receptor 1 subunit, N-terminus.
Species Reactivity
  • Rat
  • Spider
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryGamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter in the mammalian central nervous system. GABA exerts its effects through ionotropic [GABA(A/C)] receptors, to produce fast synaptic inhibition, and metabotropic [GABA(B)] receptors, to produce slow, prolonged inhibitory signals. The GABA(B) receptor consists of a heterodimer of two related 7-transmembrane receptors, GABA(B) receptor 1 and GABA(B) receptor 2. The GABA(B) receptor 1 gene is mapped to chromosome 6p21.3 within the HLA class I region close to the HLA-F gene. Susceptibility loci for multiple sclerosis, epilepsy, and schizophrenia have also been mapped in this region. Alternative splicing of this gene generates 4 transcript variants.
Gene Symbol
  • GABBR1
  • dJ271M21.1.1
  • dJ271M21.1.2
  • Gb1
  • GABA-B-R1
  • GPRC3A
  • GABBR1-3
  • GABAB(1e)
  • hGB1a
  • GABABR1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UBS5 # Isoform 1E function may be to regulate the availability of functional GABA-B-R1A/GABA-B-R2 heterodimers by competing for GABA-B-R2 dimerization. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
COFACTOR: Calcium. Required for high affinity binding to GABA.
SIZE: 961 amino acids; 108320 Da
SUBUNIT: Heterodimer of GABA-B-R1 and GABA-B-R2. Neither of which is effective on its own and homodimeric assembly does not seem to happen. Isoform 1E (without C-terminal intracellular domain) is unable to dimerize via a coiled-coil interaction with GABA-B-R2. Interacts with the leucine zipper of the C-terminal bZIP domain of ATF4 via its C-terminal region.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Note=Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Moreover coexpression of GABA-B-R1 and GABA-B-R2 appears to be a prerequisite for maturation and transport of GABA-B-R1 to the plasma membrane. & Isoform 1E: Secreted (Probable).
TISSUE SPECIFICITY: Highly expressed in brain and weakly in heart, small intestine and uterus. Mostly expressed in granular cell and molecular layer for isoform 1A and in Purkinje cells form isoform 1B. Isoform 1E is predominantly expressed in peripheral tissues as kidney, lung, trachea, colon, small intestine, stomach, bone marrow, thymus and mammary gland.
DOMAIN: SwissProt: Q9UBS5 Alpha-helical parts of the C-terminal intracellular region mediate heterodimeric interaction with GABA-B receptor 2. The linker region between the transmembrane domain 3 (TM3) and the transmembrane domain 4 (TM4) probably play a role in the specificity for G-protein coupling.
SIMILARITY: Belongs to the G-protein coupled receptor 3 family. GABA-B receptor subfamily. & Contains 2 Sushi (CCP/SCR) domains.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5850 04053252284175