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05-1341-S Sigma-AldrichAnti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size

Use Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size (Mouse Monoclonal Antibody) validated in WB, ICC, ELISA, IP, FC, DB to detect Histone H3 also known as histone 3-H3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA

Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: H3.4, H3/g , H3/t, H3FT, H3T, H3t, MGC126886, MGC126888, OTTHUMP00000037945 UniProt Number: Q16695

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Vrt	WB, ICC, ELISA, IP, FC, DB	M	Affinity Purified	Monoclonal Antibody

Description
Catalogue Number	05-1341-S
Description	Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size
Alternate Names	H3 histone family, member T Histone , H3 Histone cluster 3, H3
Background Information	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

References

Product Information
Format	Affinity Purified
Control	HeLa Acid extract lysate.
Presentation	Mouse monoclonal IgG2bκ supernatant in PBS with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Use Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size (Mouse Monoclonal Antibody) validated in WB, ICC, ELISA, IP, FC, DB to detect Histone H3 also known as histone 3-H3.
Key Applications	Western Blotting Immunocytochemistry ELISA Immunoprecipitation Flow Cytometry Dot Blot
Application Notes	ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA.1 Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1 Immunoprecipitation: This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1

Biological Information
Immunogen	Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, conjugated to KLH.
Epitope	N-terminus (unmodified)
Clone	CMA301
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Specificity	This antibody recognizes Histone H3 that is unmodified at Thr3 and Lys4 but does not recognize phosphorylated Thr3 nor acetylated or methylated Lys4. The antibody binding specificity allows modifications of Lys9, Ser10, and/or Lys141.
Isotype	IgG2bκ
Species Reactivity	Human Vertebrates
Species Reactivity Note	Human. Broad species cross-reactivity is expected, based on sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NM_003493
Entrez Gene Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Gene Symbol	H3.4 H3/g H3/t H3FT H3T H3t MGC126886 MGC126888 OTTHUMP00000037945
Purification Method	Peptide affinity chromatography
UniProt Number	Q16695
UniProt Summary	FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testicular cells. Developmental stage Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity. Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity. Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity. Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity. Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity. Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Ubiquitinated By similarity. SIMILARITY:Belongs to the histone H3 family.
Molecular Weight	Approx. 17 kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by Western Blot on HeLa cell lysate. Western Blot Analysis: 1:10,000 dilution of this antibody detected histone H3 in 10 μg of HeLa Acid Extract cell lysate and recombinant histone H3 (500 ng) but not other recombinant histones.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	10 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
05-1341-S	04053252919121

Documentation

Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size SDS

Title
Safety Data Sheet (SDS)

Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, Trial Size Certificates of Analysis

Title	Lot Number
Anti-Histone H3 (Unmodified Lys4), -2821659	2821659
Anti-Histone H3 (Unmodified Lys4), clone CMA301	2478031
Anti-Histone H3 (Unmodified Lys4), clone CMA301, Trial Size - 2537284	2537284
Anti-Histone H3 (Unmodified Lys4), clone CMA301, Trial Size - 3164200	3164200
Anti-Histone H3 (Unmodified Lys4), clone CMA301, Trial Size - 3242717	3242717
Anti-Histone H3 (Unmodified Lys4), clone CMA301, Trial Size - 3397984	3397984
Anti-Histone H3 (Unmodified Lys4), clone CMA301, Trial Size - 3784537	3784537
Anti-Histone H3 (Unmodified Lys4), clone CMA301, Trial Size - 4269951	4269951
Anti-Histone H3 (Unmodified Lys4), clone CMA301, Trial Size - 4307234	4307234
Anti-Histone H3 (Unmodified Lys4), clone CMA301, Trial Size - Q2246033	Q2246033

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies

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