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09-412 Anti-ISG15/UCRP Antibody

Detect ISG15/UCRP using this Anti-ISG15/UCRP Antibody validated for use in WB.

Anti-ISG15/UCRP Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Protein Trafficking Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: ISG15, UCRP, IFI15, G1P2 UniProt Number: P05161
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09-412
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue Number09-412
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-ISG15/UCRP Antibody
OverviewISG15 (also called ubiquitin cross-reactive protein (UCRP) due to its cross-reactivity with ubiquitin antibodies) was originally identified as an interferon stimulated gene (ISG) whose expression is highly induced upon interferon treatment. ISG15 contains two ubiquitin homology domains in tandem and shows ~30% identity to ubiquitin. Unlike ubiquitin, that is almost 100% homologous across species, ISG15 is only conserved in dispersed regions. Homology among the five identified mammalian ISG15 proteins is only about 47%. Like other Ubls, ISG15 is synthesized as a 17-kDa precursor form and processed to 15- kDa protein by a specific protease in order to expose di-glycine residues at the carboxyl terminal which is critical for subsequent conjugation to target proteins.
References
Product Information
FormatPurified
Presentation100 µL of purified by salt precipitation and peptide immunochromatography in PBS containing 0.01 M sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4 and 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used).
Quality LevelMQ100
Applications
ApplicationDetect ISG15/UCRP using this Anti-ISG15/UCRP Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenPeptide corresponding to residues 85-101 of human ISG15.
HostRabbit
SpecificityAntibody 09-412 recognizes several members of the ubiquitin-like (Ubl) family including ubiquitin, SUMO-1, SUMO-2, SUMO-3, Nedd8, FAT10, Ubl5, Urm1 and Fub1. This antibody shows no cross-reactivity with any of these Ubls in recombinant form upon Western blotting under the conditions used.
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryG1P2 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA; MIM 147660) and -beta (IFNB; MIM 147640) (Zhao et al., 2005 [PubMed 16009940]).[supplied by OMIM]
Gene Symbol
  • ISG15
  • UCRP
  • IFI15
  • G1P2
Purification MethodSalt precipitation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P05161 # In response to IFN-tau secreted by the conceptus, may ligate to and regulate proteins involved in the release of prostaglandin F2-alpha (PGF), and thus prevent lysis of the corpus luteum and maintain the pregnancy (By similarity).
SIZE: 165 amino acids; 17888 Da
SUBUNIT: Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation.
SUBCELLULAR LOCATION: Cytoplasm. Secreted. Note=UCRP conjugates seem to be noncovalently associated with the intermediate filaments and distributed in a punctate pattern. Also secreted.
TISSUE SPECIFICITY: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons.
SIMILARITY: SwissProt: P05161 ## Contains 2 ubiquitin-like domains.
Molecular Weight18 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of shipment for up to 12 months.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
09-412 04053252372339

Documentation

Anti-ISG15/UCRP Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-ISG15/UCRP Antibody Certificates of Analysis

TitleLot Number
Anti-ISG15/UCRP (Rabbit polyclonal IgG) - 1948802 1948802
Anti-ISG15/UCRP (Rabbit polyclonal IgG) - 2198965 2198965
Anti-ISG15/UCRP - 0611045512 0611045512

References

Reference overviewPub Med ID
Antiviral actions of interferons
Samuel, C E
Clin Microbiol Rev, 14:778-809, table of contents (2001)  2001

11585785 11585785
A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme
Kok, K, et al
Proc Natl Acad Sci USA, 90:6071-5 (1993)  1993

8327486 8327486