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AB5719-50UL Anti-KCa3.1 Antibody

Detect KCa3.1 using this Anti-KCa3.1 Antibody validated for use in WB.

Anti-KCa3.1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: KCNN4, hIKCa1, IK1, IKCa1, KCa4, SK4, KCA4, KCa3.1, hSK4, IKCA1, hKCa4 UniProt Number: O15554
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AB5719-50UL
50 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5719-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-KCa3.1 Antibody
Alternate Names
  • Intermediate conductance Ca2+-activated K+ Channel 4
  • SK4
  • IKCa1
  • KCNN4
References
Product Information
FormatAffinity Purified
Control
  • Included free of charge with the antibody is 40 μg of control antigen. The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 2 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Quality LevelMQ100
Applications
ApplicationDetect KCa3.1 using this Anti-KCa3.1 Antibody validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern blot: 1:300 using ECL on KCa3.1 transfected HEK-293 and K562 cells.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

SUGGESTED WESTERN BLOT PROTOCOL

1. Mix the samples (organ membranes: 50 μg/lane; transfected cells: 500,000 cells/lane) with sample-buffer X 2, and heat 10 min at 70°C.

2. 5-50 μL applied to Minigel lane (0.75-1.5 mm width) and run at standard conditions. (60 mA for 2 1.5 mm Minigel gels, 1.4 h). It is suggested that you run 5-15% acrylamide (37.5:1 acrylamide:bisacrysmide) minigel (1.5 mm width) at 30 mA/gel ~1-1.5 hours.

3. Transfer in semi-dry system under standard conditions (3 h 100 mA for two minigel gels)

4. Stain the transferred bands with Chemicon BLOT-FastStain (Catalog Number 2076).

5. Destain with deionized water.6. Block with 5% non-fat milk (Marvel or Carnation) in PBS, and 0.025 % sodium azide, overnight at 2-8°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).

7. Incubation with first antibody 2 h at room temperature or overnight at 4°C in blocking solution. The antibody preparation should be centrifuged before use (10,000 g 5 min.). Optimal working dilutions and incubation time will need to be determined by the end user.

8. Wash 4 x 10 min. with PBS-0.1% tween 20. From this stage, azide should be omitted.

9. Incubation with the secondary antibody (HRP-conjugated goat anti-rabbit antibody, for example Chemicon Catalog Number AP132P, diluted appropriately) 1 h at room temperature.

10. Wash 4 x 10 min. with PBS-0.1% tween 20.

11. Perform ECL with commercial kits (Chemilucent, Chemicon Catalog Number 2600).
Biological Information
ImmunogenSynthetic peptide from the C-terminal area of rat KCa3.1 (Accession Q9QYW1).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityKCa3.1 (Intermediate conductance Ca2+-activated K+ Channel4, IKCa1, SK4, KCNN4)
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.
Gene Symbol
  • KCNN4
  • hIKCa1
  • IK1
  • IKCa1
  • KCa4
  • SK4
  • KCA4
  • KCa3.1
  • hSK4
  • IKCA1
  • hKCa4
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O15554 # Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin.
SIZE: 427 amino acids; 47696 Da
SUBUNIT: Heterotetramer of potassium channel proteins (Probable).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Widely expressed in non-excitable tissues.
SIMILARITY: SwissProt: O15554 ## Belongs to the potassium channel KCNN family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5719-50UL 04053252320361