Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

CB1036 Anti-S100A7 Mouse mAb (1A4)

This Anti-S100A7 Mouse mAb (1A4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of S100A7.

Anti-S100A7 Mouse mAb (1A4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-S100 Calcium Binding Protein A7

View Products on Sigmaaldrich.com
CB1036
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CB1036-100UGCN 100 μg
Description
OverviewRecognizes the ~10 kDa S100A7 protein in A431 cells.
Catalogue NumberCB1036
Brand Family Calbiochem®
SynonymsAnti-S100 Calcium Binding Protein A7
Application Data
Detection of human S100A7 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human S100A7 by immunocytochemistry. Sample: A-431 cells fixed in paraformaldehyde. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: fluorescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlA-431 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human S100A7 (aa 1-102)
Clone1A4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1036-100UGCN 04055977228748

Documentation

Anti-S100A7 Mouse mAb (1A4) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-October-2010 RFH
SynonymsAnti-S100 Calcium Binding Protein A7
ApplicationELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Application Data
Detection of human S100A7 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human S100A7 by immunocytochemistry. Sample: A-431 cells fixed in paraformaldehyde. Primary antibody: Anti-S100A7 Mouse mAb (1A4) (Cat. No. CB1036) (1-5 µg/ml). Detection: fluorescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~10 kDa S100A7 protein.
BackgroundS100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein differs from the other S100 proteins of known structure in its lack of calcium binding ability in one EF-hand at the N-terminus. This protein is markedly over-expressed in the skin lesions of psoriatic patients, but is excluded as a candidate gene for familial psoriasis susceptibility. The exact function of this protein is not known.
HostMouse
ImmunogenFull-length, recombinant, human S100A7 (aa 1-102)
Clone1A4
IsotypeIgG₁
Specieshuman
Positive controlA-431 cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review