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DR1052 Anti-SALL4 Mouse mAb (6E3)

This Anti-SALL4 Mouse mAb (6E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of SALL4.

Anti-SALL4 Mouse mAb (6E3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-SAL-Like 4

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DR1052
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DR1052-100UGCN Glass bottle 100 μg
Description
OverviewRecognizes the ~100 kDa SALL4 protein in HeLa and NIH3T3 cells and human testes tissue.
Catalogue NumberDR1052
Brand Family Calbiochem®
SynonymsAnti-SAL-Like 4
Application Data
Detection of human SALL4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse SALL4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse SALL4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (0.2 µg/ml). Detection: colorimetric (DAB).
References
ReferencesCauffman, G., et al. 2009. Hum. Reprod. 24 63.
Forte, A., et al. 2009. Mol. Med. 15 392.
Product Information
Formliquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, NIH3T3 cells, Human testes tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (0.2 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
ImmunogenNA
Clone6E3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1052-100UGCN 04055977225983

Documentation

Anti-SALL4 Mouse mAb (6E3) SDS

Title

Safety Data Sheet (SDS) 

Anti-SALL4 Mouse mAb (6E3) Certificates of Analysis

TitleLot Number
DR1052

References

Reference overview
Cauffman, G., et al. 2009. Hum. Reprod. 24 63.
Forte, A., et al. 2009. Mol. Med. 15 392.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-August-2015 JSW
SynonymsAnti-SAL-Like 4
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (0.2 µg/ml)
Application Data
Detection of human SALL4 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse SALL4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (1-5 µg/ml). Detection: chemiluminescence.

Detection of mouse SALL4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-SALL4 Mouse mAb (6E3) (Cat. No. DR1052) (0.2 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.
BackgroundThis protein may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS).
HostMouse
Immunogen speciesNA
ImmunogenA recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
Clone6E3
IsotypeIgG₁
Specieshuman, mouse
Positive controlHeLa cells, NIH3T3 cells, Human testes tissue
Formliquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesCauffman, G., et al. 2009. Hum. Reprod. 24 63.
Forte, A., et al. 2009. Mol. Med. 15 392.