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ABT13 Sigma-AldrichAnti-Sarcolipin Antibody

Anti-Sarcolipin, Cat. No. ABT13, is a rabbit polyclonal antibody that detects Sarcolipin and is tested for use in Peptide Inhibition Assay and Western Blotting.

Anti-Sarcolipin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Brochures

Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: SLN UniProt Number: O00631

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
Rb	WB, PIA	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	ABT13
Description	Anti-Sarcolipin Antibody
Alternate Names	Sarcopolin
Background Information	Sarcolipin (UniProt: O00631; also known as SLN) is encoded by the SLN gene (Gene ID: 6588) in human. Sarcolipin is a single-pass membrane protein that contains a cytoplasmic domain (aa 1-7), a transmembrane domain (aa 8-26), and a lumenal domain (aa 27-31). SLN is a regulator of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) activity in muscle and plays a role in regulating muscle thermogenesis and metabolism. It increases the heat generated by SERCA by partially uncoupling Ca2+ re-sequestration from ATP hydrolysis in muscle and contributes to non-shivering thermogenesis. This heat generating process is mechanistically distinct from and unrelated to Ca2+-linked thermogenic processes in skeletal muscle. It acts by reducing the apparent affinity of the ATPase for Ca2+. SLN is also reported to modulate calcium re-uptake during muscle relaxation and play an important role in calcium homeostasis in muscle. Higher levels of SLN have been reported in patients with Duchenne muscular dystrophy and reducing SLN levels improves dystrophic pathology. (Ref.: Campbell, KL., and Dicke, AA (2018). Front. Physiol. 9; Article 714; Voit, A., et al. (2017). Nat. Commun. 8; Article 1068).

References

Product Information
Format	Affinity Purified
HS Code	3002 15 90
Control	Rabbit quadraceps tissue
Presentation	Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	Anti-Sarcolipin, Cat. No. ABT13, is a rabbit polyclonal antibody that detects Sarcolipin and is tested for use in Peptide Inhibition Assay and Western Blotting.
Key Applications	Western Blotting Peptide Inhibition Assay
Application Notes	Peptide Inhibition Assay: Sarcolipin detection in Rabbit Quadricept tissue lysate was completely inhibited by preblocking of this antibody with 1 mg of antigen peptide for 1 hour at 37⁰C . Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to the C-terminus of human Sarcolipin.
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	This rabbit polyclonal antibody detects Sarcolipin in multiple mammalian species. It targets an epitope within 6 amino acids from the C-terminal lumenal domain.
Species Reactivity	Rabbit
Species Reactivity Note	Rabbit. Predicted to react with Bovine, Human, Mouse, Monkey, Rat based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_003054
Entrez Gene Summary	Sarcoplasmic reticulum Ca(2+)-ATPases are transmembrane proteins that catalyze the ATP-dependent transport of Ca(2+) from the cytosol into the lumen of the sarcoplasmic reticulum in muscle cells. This gene encodes a small proteolipid that regulates several sarcoplasmic reticulum Ca(2+)-ATPases. The transmembrane protein interacts with Ca(2+)-ATPases and reduces the accumulation of Ca(2+) in the sarcoplasmic reticulum without affecting the rate of ATP hydrolysis.
Gene Symbol	SLN
Purification Method	Affinity Purfied
UniProt Number	O00631
UniProt Summary	SUBUNIT STRUCTURE: Associated with calcium ATPase SERCA1. SUBCELLULAR LOCATION: Sarcoplasmic reticulum membrane; Single-pass membrane protein. SEQUENCE SIMILARITIES: Belongs to the sarcolipin family.
Molecular Weight	~6 kDa observed; 3.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in Rabbit Quadriceps muscle tissue lysate. Western Blotting Analysis: A 1:500 dilution of this antibody detected Sarcolipin in Rabbit Quadriceps muscle tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
ABT13	04053252644450

Documentation

Anti-Sarcolipin Antibody SDS

Title
Safety Data Sheet (SDS)

Anti-Sarcolipin Antibody Certificates of Analysis

Title	Lot Number
Anti-Sarcolipin - 3013471	3013471
Anti-Sarcolipin - 3143173	3143173
Anti-Sarcolipin - 3537160	3537160
Anti-Sarcolipin - 3598057	3598057
Anti-Sarcolipin - 3684752	3684752
Anti-Sarcolipin - 3768101	3768101
Anti-Sarcolipin - 3866324	3866324
Anti-Sarcolipin - 3928032	3928032
Anti-Sarcolipin - 4065423	4065423
Anti-Sarcolipin -2622046	2622046

References

Reference overview	Pub Med ID
The effects of sarcolipin over-expression in mouse skeletal muscle on metabolic activity. Butler, J; Smyth, N; Broadbridge, R; Council, CE; Lee, AG; Stocker, CJ; Hislop, DC; Arch, JR; Cawthorne, MA; Malcolm East, J Archives of biochemistry and biophysics 569 26-31 2015 Show Abstract Studies in sarcolipin knockout mice have led to the suggestion that skeletal muscle sarcolipin plays a role in thermogenesis. The mechanism proposed is uncoupling of the sarcoplasmic reticulum calcium pump. However, in other work sarcolipin was not detected in mouse skeletal tissue. We have therefore measured sarcolipin levels in mouse skeletal muscle using semi-quantitative western blotting and synthetic mouse sarcolipin. Sarcolipin levels were so low that it is unlikely that knocking out sarcolipin would have a measurable effect on thermogenesis by SERCA. In addition, overexpression of neither wild type nor FLAG-tagged variants of mouse sarcolipin in transgenic mice had any major significant effects on body mass, energy expenditure, even when mice were fed on a high fat diet.	25660043

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