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MABN1193 Anti-Synaptophysin Antibody, clone 10F6.1

Anti-Synaptophysin Antibody, clone 10F6.1, Cat. No. MABN1193, is a highly specific monoclonal antibody that targets Synaptophysin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Synaptophysin Antibody, clone 10F6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Syp UniProt Number: P07825
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MABN1193
50 µL  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1193
ReplacesMAB368
DescriptionAnti-Synaptophysin Antibody, clone 10F6.1
Alternate Names
  • Major synaptic vesicle protein p38
Background InformationSynaptophysin (UniProt: P07825; also known as Major synaptic vesicle protein p38) is encoded by the Syp gene (Gene ID: 24804) in rat. Synaptophysin belongs to the synaptophysin/synaptobrevin family of proteins. It is one of the major glycoproteins present in presynaptic vesicles and in various neuroendocrine cells of both neuronal and epithelial phenotype. Its structure consists of a hexameric protein of four transmembrane spans with a short cytoplasmic amino and a long carboxy-terminal tail. Both amino- and carboxy-terminals are exposed on the cytoplasmic surface of the synaptic vesicle membrane. C-terminal domain of Snaptophysin is selectively required for the endocytosis during, but not after, cessation of sustained synaptic transmission. Synaptophysin is involved in organizing membrane components may regulate endocytosis to ensure vesicle availability following sustained neuronal activity. It is also involved in the regulation of short-term and long-term synaptic plasticity. Syp / neurons display functional deficiencies and slower recovery of the recycling synaptic vesicle pool. (Ref.: Kwon, SE and Chapman, ER (2011). Neuron. 70(5): 847 854).
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Synaptophysin Antibody, clone 10F6.1, Cat. No. MABN1193, is a highly specific monoclonal antibody that targets Synaptophysin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Synaptophysin in human cerebellum, human cerebral cortex, and human pancreatic tissues.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corrsponding to 86 amino acids from the C-terminal region of rat synaptophysin.
Clone10F6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 10F6.1 detects synaptophysin in rat, human, and mouse brain. It targets a sequence within amino acids 222-307 in rat synaptophysin.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Syp
Purification MethodProtein G purified
UniProt Number
Molecular Weight~40 kDa observed; 33.31 kDa calculated. Glycosylated form displays higher molecular weight (~38 kDa). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected Synaptophysin in 10 µg of rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1193 04054839169595

Documentation

Anti-Synaptophysin Antibody, clone 10F6.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-Synaptophysin Antibody, clone 10F6.1 Certificates of Analysis

TitleLot Number
Anti-Synaptophysin, clone 10F6.1 - 3438585 3438585
Anti-Synaptophysin, clone 10F6.1 - 3766689 3766689
Anti-Synaptophysin, clone 10F6.1 - 3850550 3850550
Anti-Synaptophysin, clone 10F6.1 - 3870886 3870886
Anti-Synaptophysin, clone 10F6.1 - 3915554 3915554
Anti-Synaptophysin, clone 10F6.1 - 4264852 4264852
Anti-Synaptophysin, clone 10F6.1 -Q2814934 Q2814934
Anti-Synaptophysin, clone 10F6.1 Monoclonal Antibody 3055402
Anti-Synaptophysin, clone 10F6.1 Monoclonal Antibody 2941469