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AP1168 Anti-TNFSF14 Mouse mAb (4E3)

This Anti-TNFSF14 Mouse mAb (4E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of TNFSF14.

Anti-TNFSF14 Mouse mAb (4E3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tumor Necrosis Factor Receptor Like 2, Anti-Tumor Necrosis Factor Superfamily Member 14

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AP1168
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
AP1168-100UGCN 100 μg
Description
OverviewRecognizes the TNFSF14 protein.
Catalogue NumberAP1168
Brand Family Calbiochem®
SynonymsAnti-Tumor Necrosis Factor Receptor Like 2, Anti-Tumor Necrosis Factor Superfamily Member 14
Application Data
Detection of human TNFSF14 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TNFSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (3 µg/ml). Detection: colorimetric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHuman spleen tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 61-170 human TNFSF14, expressed as a GST fusion protein
Clone4E3
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1168-100UGCN 04055977212693

Documentation

Anti-TNFSF14 Mouse mAb (4E3) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-October-2010 JLN
SynonymsAnti-Tumor Necrosis Factor Receptor Like 2, Anti-Tumor Necrosis Factor Superfamily Member 14
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human TNFSF14 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human TNFSF14 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human spleen tissue. Primary antibody: Anti-TNFSF14 Mouse mAb (4E3) (Cat. No. AP1168) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the TNFSF14 protein.
BackgroundTNFSF14 is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, a member of the tumor necrosis factor receptor superfamily, whereby TNFRSF14 is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 61-170 human TNFSF14, expressed as a GST fusion protein
Clone4E3
IsotypeIgG2a
Specieshuman
Positive controlHuman spleen tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review