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MABN750 Anti-VANGL2 Antibody, clone 2G4

Anti-VANGL2 Antibody, clone 2G4, Cat. No. MABN750, is a highly specific rat monoclonal antibody that targets VANGL2 and has been tested in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-VANGL2 Antibody, clone 2G4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Vangl2, Lpp1, Ltap, Stb1, Stbm UniProt Number: Q91ZD4
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MABN750
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, ELISA, IF, IPRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN750
DescriptionAnti-VANGL2 Antibody, clone 2G4
Alternate Names
  • Vang-like protein 2
  • Loop-tail-associated protein
  • Loop-tail protein 1
  • Strabismus 1
  • Van Gogh-like protein 2
Background InformationVang-like protein 2 (UniProt Q91ZD4; also known as Loop-tail-associated protein, Loop-tail protein 1, Van Gogh-like protein 2) is encoded by the Vangl2 (also known as Lpp1, Ltap, Stb1, Stbm) gene (Gene ID 93840) in murine species. Mammalian Van Gogh-like proteins (VANGL1 & VANGL2) are orthologues of Vangl/Strabismus originally identified as a core planar-cell-polarity (PCP) gene in Drosophila. VANGL1 & VANGL2 are proteins of the WNT/PCP pathway that acts as a key regulator of cell polarity and directional movements that plays a key role in tissue morphogenesis and embryonic development. PCP is involved in neural tube closure, in the orientation of hair bundle in inner ear sensory cells and of motile cilia in the embryonic node, as well as in asymmetric cell division. VANGL1 and VANGL2 missense mutations have been identified in human embryos affected with severe neural tube defects (NTD). Likewise, homozygous Vangl2 Looptail mutation (Lp) in mice causes morphogenesis and patterning defects in numerous tissues, including the most severe failure in neural tube closure (craniorachischisis). VANGL1 and VANGL2 are both 4-transmembrane (a.a. 109-129, 148-168, 179-199, and 218-238 of murine VANGL2) plasma membrane proteins with cytoplasmic N- and C-terminal ends (a.a. 1-108 and 239-521 of murine VANGL2), they show overlapping, but not identical expression patterns in mouse brain. Both proteins can homo- or heterodimerize, creating different complexes during embryonic development.
References
Product Information
FormatPurified
PresentationPurified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-VANGL2 Antibody, clone 2G4, Cat. No. MABN750, is a highly specific rat monoclonal antibody that targets VANGL2 and has been tested in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
  • Immunofluorescence
  • Immunoprecipitation
Application NotesELISA Analysis: Clone 2G4 hybridoma culture supernatant specifically detected VANGL2, but not VANGL1, N-terminal fragment GST fusion (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Immunofluorescence Analysis: A representative lot detected VANGL2 immunoreactivity co-localized with that of VANGL1 at the cell membrane of the stereociliary hair bundles by fluorescent immunohistochemistry staining of 4% paraformaldehyde-fixed cochleae whole mount sections. A drastically reduced VANGL2 was observed in tissue sections from homozygous Vangl2 Looptail mutant (Lp/Lp) mice (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Immunoprecipitation Analysis: A representative lot immunoprecipitated VANGL2, but not VANGL1, N-terminal fragment GST fusion, as well as endogenous VANGL2 from untreated, but not VANGL2 shRNA-treated SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Western Blotting Analysis: A representative lot detected the endogenous VANGL2 as well as exogenously expressed yellow fluorescent protein Venus-tagged VANGL2 in transfected in MEC1 human chronic lymphocytic leukemia (CLL) cells (Kaucká, M., et al. (2015). Cell Commun Signal. 13:2).

Western Blotting Analysis: A representative lot detected VANGL2, but not VANGL1, N-terminal fragment GST fusion, as well as endogenous VANGL2 in lysate from untreated, but not VANGL2 shRNA-treated SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).

Western Blotting Analysis: A representative lot detected higher VANGL2 expression in mouse brain and lung than kidney. A decreased cochlear VANGL2 expression was found among mice with heterozygous Vangl2 Looptail mutation (Lp), the decrease was even more pronounced among homozygous Vangl2(Lp/Lp) mice (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
Biological Information
ImmunogenGST-tagged recombinant VANGL2 N-terminal fragment 100% conserved among human, mouse, and rat species.
Clone2G4
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 2G4 reacted specifically with VANGL2, but not VANGL1. Clone 2G4 detected shRNA-mediated VANGL2 downregulation in SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine and Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Vangl2
  • Lpp1
  • Ltap
  • Stb1
  • Stbm
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~60 kDa observed. 59.71 kDa (human) and 59.77 kDa (mouse & rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected VANGL2 in 10 µg of mouse brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN750 04054839069161

Documentation

Anti-VANGL2 Antibody, clone 2G4 SDS

Title

Safety Data Sheet (SDS) 

Anti-VANGL2 Antibody, clone 2G4 Certificates of Analysis

TitleLot Number
Anti-VANGL2, clone 2G4 - 3215510 3215510
Anti-VANGL2, clone 2G4 - 3379913 3379913
Anti-VANGL2, clone 2G4 - 3527699 3527699
Anti-VANGL2, clone 2G4 - 3596737 3596737
Anti-VANGL2, clone 2G4 - 3744283 3744283
Anti-VANGL2, clone 2G4 - 3794319 3794319
Anti-VANGL2, clone 2G4 - 3984020 3984020
Anti-VANGL2, clone 2G4 - 3991442 3991442
Anti-VANGL2, clone 2G4 - 4139153 4139153
Anti-VANGL2, clone 2G4 -Q2753956 Q2753956
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