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14-511M Aurora A Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant full-length human Aurora A, for use in Kinase Assays.

Aurora A Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-511M
250 µg  Also available in 10 μg size (cat#14-511) and in bulk (cat# 14-511-K).
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Overview

Replacement Information
Description
Catalogue Number14-511M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAurora A Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant full-length human Aurora A
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged, recombinant full-length human Aurora A, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • AURKA
  • STK7
  • AURA
  • AIK
  • hARK1
  • BTAK
  • AURORA2
  • aurora-A
  • Aurora-A
  • STK15
  • AurA
  • ARK1
  • MGC34538
  • STK6
Protein TargetAurora-A
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14965 # May play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Maybe involved in microtubule formation and/or stabilization. May play a key role during tumor development and progression.
SIZE: 403 amino acids; 45809 Da
SUBUNIT: Interacts with TACC1 and CPEB1. Interacts with and phosphorylates BORA.
SUBCELLULAR LOCATION: Centrosome. Spindle. Note=Localizes on centrosomes in interphase cells and at each spindle pole in mitosis.
TISSUE SPECIFICITY: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly after.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.DISEASE:SwissProt: O14965 # Defects in AURKA are responsible for numerical centrosome aberrations including aneuploidy.
SIMILARITY: SwissProt: O14965 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. & Contains 1 protein kinase domain.
Molecular Weight46.9kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Kemptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-511) and in bulk (cat# 14-511-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-511M 04053252586828

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-511 Aurora A Protein, active, 10 µg

Product Families

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Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases