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PF126 Bcl-2, C-Terminal Truncated, His•Tag®, Human, Recombinant, E. coli

Recombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag® sequence, and expressed in E. coli.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PF126
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
PF126-50UGCN Glass bottle 50 μg
Description
OverviewRecombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His•Tag® sequence, and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.
Catalogue NumberPF126
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationIn 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.
Quality LevelMQ100
Applications
Key Applications
Application NotesNeutralization (see biological activity)
Biological Information
Biological activityEC₅₀ = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria
Purity≥95% by SDS-PAGE
Species Reactivity
  • Human
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PF126-50UGCN 04055977226904

Documentation

Bcl-2, C-Terminal Truncated, His•Tag®, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Bcl-2, C-Terminal Truncated, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
PF126

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-September-2008 RFH
ApplicationNeutralization (see biological activity)
DescriptionRecombinant, human Bcl-2 without the 17 amino acid mitochondrial targeting sequence at the C-terminus, fused to a His-Tag® sequence and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.
BackgroundHuman recombinant protein Bcl-2, minus the 17 amino acid mitochondrial targeting sequence at the C-terminus, was fused to a His-Tag® and expressed in E. coli. The truncated protein retains the ability to neutralize pro-apoptotic Bcl-2 family members.
Specieshuman
FormLiquid
FormulationIn 100 mM KCl, 10 mM HEPES, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Biological activityEC₅₀ = 100-300 nM based on its ability to inhibit 54 nM caspase-8-cleaved human Bid-mediated release of cytochrome c from isolated mouse liver mitochondria
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling