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14-476M Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 250 µg

Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.

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14-476M
250 µg  Also available in 10 μg size (cat#14-476) and in bulk (cat# 14-476-K).
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Overview

Replacement Information
Description
Catalogue Number14-476M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 250 µg
OverviewComplex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceAll are expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.
Gene Symbol
  • CDK7
  • p39MO15
  • CAK
  • CAK1
  • MO15
  • CDKN7
  • STK1
Protein TargetCDK7/cyclinH/MAT1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P50613 # Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
SIZE: 346 amino acids; 39038 Da
SUBUNIT: Associates primarily with cyclin H and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor. Interacts with PUF60.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Phosphorylation of Ser-164 during mitosis inactivates the enzyme. & Phosphorylation of Thr-170 is required for activity.
SIMILARITY: SwissProt: P50613 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight39.9kDa (cdk7), 37.8kDa (cyclin H), 62.8kDa (MAT1).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of cdk7 substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-476) and in bulk (cat# 14-476-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-476M 04053252678752

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-476 Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases