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109844 Sigma-AldrichEosin Y-solution 0.5% aqueous

for microscopy

Eosin Y-solution 0.5% aqueous MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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Catalogue Number	Packaging	Qty/Pack
1.09844.1000	Plastic bottle	1 l	Add To Favorites Add To Favorites Added To My Favorites

Description
Catalogue Number	109844
Description	Eosin Y-solution 0.5% aqueous
Overview	Eosin Y-solution 0.5% aqueous - for microscopy, is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin. The hematoxylin and eosin (H&E) staining method is the method most frequently used for the staining of histology material. This Eosin Y-solution is a 0.5% aqueous staining solution, that can be used e. g. for the histological staining of instantaneous sections.The staining mechanism behind the H&E staining is a physico-chemical process. In the first step, the positively charged nuclear dye (hematoxylin) Mayer’s hemalum solution for microscopy(Cat.no. 109249) or Hematoxylin solution modified acc. to Gill III (Cat. no. 105174) binds to the negatively charged phosphate groups of the nucleic acid of the cell nucleus. The nuclei will be dyed dark blue to dark violet. The second step is the counterstaining with negatively charged anionic Eosin Y, a xanthene dye. Eosin binds to the positively charged plasma proteins. Cytoplasm and intercellular substances are stained pink to red, while erythrocytes will appear with yellow to orange color. The 500ml bottle provides 1500 stainings. It is an IVD registered product and CE certified, thus can be used for clinical diagnostic purposes. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

References

Product Information
HS Code	3212 90 90
Quality Level	MQ400

Applications
Application	for microscopy

Biological Information

Physicochemical Information
Density	1.00 g/cm3

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H317: May cause an allergic skin reaction.
Precautionary Statement(s)	P261: Avoid breathing mist or vapours. P272: Contaminated work clothing should not be allowed out of the workplace. P280: Wear protective gloves. P302 + P352: IF ON SKIN: Wash with plenty of water. P333 + P313: If skin irritation or rash occurs: Get medical advice/ attention. P362 + P364: Take off contaminated clothing and wash it before reuse.
Signal Word	Warning
Storage class	10 - 13 Other liquids and solids
WGK	WGK 2 obviously hazardous to water
Disposal	28 Aqueous solutions: Container D.

Safety Information

Product Usage Statements

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Packaging Information

Transport Information

Supplemental Information

Specifications
Suitability for microscopy (Tissue section)	passes test
nuclei	dark blue to dark violet
Cytoplasm	pink to red
intercellular substances	pink to red
Erythrocytes	yellow to orange

Global Trade Item Number
Catalogue Number	GTIN
1.09844.1000	04022536557625

Documentation

Eosin Y-solution 0.5% aqueous SDS

Title
Safety Data Sheet (SDS)

Eosin Y-solution 0.5% aqueous Certificates of Analysis

Product Number	Packaging	Specification	Lot Number
1.09844.1000	Plastic bottle	PDF Specification Document	Enter Lot Number

User Guides

Title
User Guide (IFU) - 109844

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Categories

IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Solutions

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