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109204 Sigma-AldrichGiemsa's azur eosin methylene blue solution

for microscopy

Giemsa's azur eosin methylene blue solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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Catalogue Number	Packaging	Qty/Pack
1.09204.0100	Glass bottle	100 ml	Add To Favorites Add To Favorites Added To My Favorites
1.09204.0500	Plastic bottle	500 ml	Add To Favorites Add To Favorites Added To My Favorites
1.09204.1000	Plastic bottle	1 l	Add To Favorites Add To Favorites Added To My Favorites
1.09204.2500	Plastic bottle	2.5 l	Add To Favorites Add To Favorites Added To My Favorites
1.09204.9025	Barrel PE/met	25 l	Add To Favorites Add To Favorites Added To My Favorites

Description
Catalogue Number	109204
Description	Giemsa's azur-eosin-methylene blue solution
Overview	Giemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

References

Product Information
HS Code	3212 90 00
Quality Level	MQ400

Applications
Application	for microscopy

Biological Information

Physicochemical Information
Boiling point	>65 °C (1013 hPa)
Density	0.99 g/cm3 (20 °C)
Explosion limit	5.5 - 44 %(V) Methanol
Flash point	18 °C
Ignition temperature	455 °C
pH value	6.0 - 8.2 (H₂O, 20 °C) (undiluted)

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
Hazard Pictogram(s)
Hazard Statement(s)	H225: Highly flammable liquid and vapour. H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled. H317: May cause an allergic skin reaction. H370: Causes damage to organs.
Precautionary Statement(s)	P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking. P233: Keep container tightly closed. P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection. P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor. P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water. P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor. P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal Word	Danger
Storage class	3 Flammable liquids
WGK	WGK 2 obviously hazardous to water
Disposal	9 Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).

Safety Information
Categories of danger	highly flammable, toxic

Product Usage Statements

Storage and Shipping Information
Storage	Store at +15°C to +25°C.

Packaging Information

Transport Information
Declaration (railroad and road) ADR, RID	UN 1230 , 3 (6.1), II
Declaration (transport by air) IATA-DGR	UN 1230 , 3 (6.1), II
Declaration (transport by sea) IMDG-Code	UN 1230 , 3 (6.1), II

Supplemental Information

Specifications
Suitability for microscopy	passes test
Erythrocytes	reddish-brownish
Nuclei / Chromatin	red to violet
Eosinophilic granules	reddish to red-brown
Neutrophilic granules	light violet
Lymphocyte cytoplasmn	blue
Monocyte cytoplasm	grey-blue
Thrombocytes	violet
Basophilic granules	dark violet
Nuclei (Tissue section)	blue to dark blue
Cells	blue to dark blue
Collagen	pale blue
Osteoid	pale blue
Eosinophilic granula (Tissue section)	red
Acidic mucopolysaccharides	reddish-violet
Mast cell granules	reddish-violet
Cartilage matrix	reddish-violet
Acidophilic materials	orange-red
Helicobacter pylori	blue to dark blue
	blue to dark blue

Global Trade Item Number
Catalogue Number	GTIN
1.09204.0100	04022536111841
1.09204.0500	04022536111889
1.09204.1000	04022536720081
1.09204.2500	04022536111926
1.09204.9025	04022536111940

Documentation

Giemsa's azur eosin methylene blue solution SDS

Title
Safety Data Sheet (SDS)

Giemsa's azur eosin methylene blue solution Certificates of Analysis

Product Number	Packaging	Specification	Lot Number
1.09204.0100	Glass bottle	PDF Specification Document	Enter Lot Number
1.09204.0500	Plastic bottle	PDF Specification Document	Enter Lot Number
1.09204.1000	Plastic bottle	PDF Specification Document	Enter Lot Number
1.09204.2500	Plastic bottle	PDF Specification Document	Enter Lot Number
1.09204.9025	Barrel PE/met	PDF Specification Document	Enter Lot Number

User Guides

Title
User Guide (IFU) - 109204

Catalogue Number	Description
109203	Giemsa's azur-eosin-methylene blue

Accessories (Primary)

Catalogue Number	Description
101424	May-Grünwald's eosine-methylene blue solution modified
101728	OSTEOSOFT®
101736	OSTEOMOLL®
106009	Methanol
109016	Neo-Mount™
109468	Buffer tablets pH 7.2
109634	2-Propanol
109843	Neo-Clear™
111374	Buffer tablets pH 6.8

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109204 Sigma-AldrichGiemsa's azur eosin methylene blue solution

for microscopy

Recommended Products

Overview

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Giemsa's azur eosin methylene blue solution SDS

Giemsa's azur eosin methylene blue solution Certificates of Analysis

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