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12-421 MYPT1, (714-1004)

MYPT1, (714-1004) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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12-421
50 µg  
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Overview

Replacement Information
Description
Catalogue Number12-421
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMYPT1, (714-1004)
References
Product Information
Presentation50mM Tris-HCl pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF and 50% glycerol
Quality LevelMQ100
Applications
Key Applications
  • Kinase Assay
Biological Information
Entrez Gene Number
Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase.
Gene Symbol
  • PPP1R12A
  • MYPT1
  • MGC133042
  • MBS
Protein TargetROKα/ROCK-II
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.
SIZE: 1030 amino acids; 115281 Da
SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.
SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.
PTM: Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: O14974 ## Contains 6 ANK repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated as a substrate for ROKa/ROCK-II, active.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
12-421 04053252408618

Documentation

MYPT1, (714-1004) Certificates of Analysis

TitleLot Number
MYPT1 (714-1004) 2477877
MYPT1 (714-1004) - 2457109 2457109
MYPT1 (714-1004) (recombinant protein expressed in E. coli) 2947252
MYPT1 (714-1004) (recombinant protein expressed in E. coli) -2492433 2492433
MYPT1 (714-1004) - 2533234 2533234
MYPT1 (714-1004) - 2876675 2876675
MYPT1 (714-1004) - 2998339 2998339
MYPT1 (714-1004) - DAM1606765 DAM1606765
MYPT1 (714-1004) - DAM1651176 DAM1651176
MYPT1, (714-1004) - 20305 20305
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