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14-804 Met (Y1248H) Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human, Met amino acids 974-end, containing the mutation Y1248H. For use in Kinase Assays.

Met (Y1248H) Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-804
10 µg  Available in 250 μg --call for pricing and availability and reference catalog number 14-804M when ordering the 250 μg size.
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-804
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMet (Y1248H) Protein, active, 10 µg
Overview N-terminal 6His-tagged, recombinant, human, Met amino acids 974-end, containing the mutation Y1248H.
Background InformationMet Y1248H is a somatic mutation that has been identified in patients with papillary renal carcinomas (HPRC). In vitro studies have demonstrated enhanced kinase activity from this mutant.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human, Met amino acids 974-end, containing the mutation Y1248H. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • HGFR
  • RCCP2
  • c-Met
  • AUTS9
  • MET
Protein TargetMet
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08581 # Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity.
SIZE: 1390 amino acids; 155527 Da
SUBUNIT: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P08581 # Activation of MET after rearrangement with the TPR gene produces an oncogenic protein. & Defects in MET may be associated with gastric cancer. & Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550]. & Defects in MET are a cause of hereditary papillary renal carcinoma (HPRC) [MIM:605074]; also known as papillary renal cell carcinoma 2 (RCCP2). HPRC is a form of inherited kidney cancer characterized by a predisposition to develop multiple, bilateral papillary renal tumors. The pattern of inheritance is consistent with autosomal dominant transmission with reduced penetrance. & Genetic variations in MET may be associated with susceptibility to autism type 1B (AUTS1B) [MIM:608636]. Autism is a neurodevelopmental disorder characterized by disturbance in language, perception and socialization. The disorder is classically defined by a triad of limited or absent verbal communication, a lack of reciprocal social interaction or responsiveness, and restricted, stereotypical, and ritualized patterns of interests and behavior.
SIMILARITY: SwissProt: P08581 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. & Contains 3 IPT/TIG domains. & Contains 1 protein kinase domain. & Contains 1 Sema domain.
Molecular Weight50.1 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAvailable in 250 μg --call for pricing and availability and reference catalog number 14-804M when ordering the 250 μg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-804 04053252619328

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Alternative Packsize

Catalogue Number Description
14-804M Met (Y1248H) Protein, active, 250 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases