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14-444 PKC θ Protein, active, 10 µg

Active, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays.

PKC θ Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-444
10 µg  
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Overview

Replacement Information
Description
Catalogue Number14-444
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKC θ Protein, active, 10 µg
OverviewRecombinant full-length human PKC theta with a C-terminal 6His-tag

PKC Lipid activator (Catalog # 20-133) is required for use.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant full-length human PKC theta with a C-terminal 6His-tag. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
Gene Symbol
  • PRKCQ
  • OTTHUMP00000043365
  • MGC126514
  • OTTHUMP00000043364
  • MGC141919
  • PRKCT
  • nPKC-theta
Protein TargetPKCθ
Purification Methodimmobilized nickel affinity chromatography
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q04759 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
COFACTOR: Magnesium.
SIZE: 706 amino acids; 81865 Da
SUBUNIT: Interacts with TXNL2/PICOT.
TISSUE SPECIFICITY: Skeletal muscle, megakaryoblastic cells and platelets.
DOMAIN: SwissProt: Q04759 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
PTM: Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
Molecular Weight83kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Histone H1 substrate in vitro
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70 °C
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-444 04053252270130

Documentation

PKC θ Protein, active, 10 µg SDS

Title

Safety Data Sheet (SDS) 

PKC θ Protein, active, 10 µg Certificates of Analysis

TitleLot Number
PKC Theta, active - D7MN009U-H D7MN009U-H
PKC Theta, active - D7MN009U-I D7MN009U-I
PKC Theta, active - D7MN010U-D D7MN010U-D

References

Reference overviewApplicationPub Med ID
Complex formation and cooperation of protein kinase C theta and Akt1/protein kinase B alpha in the NF-kappa B transactivation cascade in Jurkat T cells
Bauer, B., et al
J Biol Chem, 276:31627-34 (2001)  2001

Kinase Assay11410591 11410591
Molecular cloning and characterization of PKC theta, a novel member of the protein kinase C (PKC) gene family expressed predominantly in hematopoietic cells.
Baier, G, et al.
J. Biol. Chem., 268: 4997-5004 (1993)  1993

8444877 8444877

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Alternative Packsize

Catalogue Number Description
14-444M PKC θ Protein, active, 250 µg

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Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases