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14-352M Raf-1 Protein, Active, 250 µg

Active, N-terminal GST-tagged recombinant human Raf-1 residues 306-end, containing the mutations Y340D & Y341D, for use in Kinase Assays.

Raf-1 Protein, Active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-352M
250 µg  Also available in 10 μg size (cat#14-352) and in bulk (cat# 14-352-K).
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Overview

Replacement Information
Description
Catalogue Number14-352M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionRaf-1 Protein, Active, 250 µg
OverviewN-terminal GST-tagged recombinant human Raf-1 residues 306-end, containing the mutations Y340D and Y341D
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal GST-tagged recombinant human Raf-1 residues 306-end, containing the mutations Y340D & Y341D, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryRaf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. [Contributed text]
Gene Symbol
  • RAF1
  • C-RAF
  • cRaf
  • Raf-1
  • c-Raf
  • CRAF
  • RAF
Protein Targetc-RAF
Purification MethodGlutathione agarose affinity chromatography
Target Sub-FamilyTKL
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04049 # Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
COFACTOR: Binds 2 zinc ions per subunit.
SIZE: 648 amino acids; 73052 Da
SUBUNIT: Interacts with Ras proteins. The interaction with Ras is antagonized by RIN1. Weakly interacts with RIT1 (By similarity). Interacts with STK3, which inhibits its pro-apoptotic activity.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY:SwissProt: P04049 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. & Contains 1 phorbol-ester/DAG-type zinc finger. & Contains 1 protein kinase domain. & Contains 1 RBD (Ras-binding) domain.
Molecular Weight65kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP (Catalog # 13-104) substrate in vitro
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-352) and in bulk (cat# 14-352-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-352M 04053252738654

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-352 Raf-1 Protein, Active, 10 µg

Product Families

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Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases