Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCC234 YUMM2.2 Mouse Melanoma Cell Line

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM2.2 harbors a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis and inactivates wt Pten and Cdkn2a.

SCC234
>1x10^6 cells/vial  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCC234
DescriptionYUMM2.2 Mouse Melanoma Cell Line
OverviewThe YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM2.2 cells are syngeneic with the immunocompetent C57Bl/6 mouse background.
Alternate Names
  • Yale University Mouse Melanoma cell line
  • Yale University Mouse Melanoma 2.2 cell line
Background InformationThe hope for immune-based therapies in cancer and recent progress in successful application of these approaches has focused research on the necessity for immunocompetent models to assess immune system responses to cancer cells. Immunocompetent genetically-engineered mouse models harboring discrete genetic drivers of melanoma are essential for studying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.
The YUMM2.2 cell line harbors a stabilized beta-catenin allele from excision of exon 3, a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis, enhances metastasis, and controls tumor differentiation. Exon 3 excision is analogous to human beta-catenin stabilizing mutations found in human melanomas.

Source
The YUMM2.2 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically engineered mouse model had been introduced via backcrossing.

References:
1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.
2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.
References
Product Information
Components
  • ≥1x106 viable YUMM2.2 Cells: (Catalog No. SCC234). Store in liquid nitrogen.
Quality LevelMQ100
Applications
ApplicationThe YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM2.2 harbors a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis and inactivates wt Pten and Cdkn2a.
Key Applications
  • Cell Culture
Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
Biological Information
HostMouse
Cell Line Type
  • Cancer Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
• YUMM2.2 cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for interspecies contamination from rat, chinese hamster, Golden Syrian hamster, human and nonhuman primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Storage and Shipping Information
Storage ConditionsYUMM2.2 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting cell marker expression and functionality.
Packaging Information
Material Size>1x10^6 cells/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC234 04065270885036