Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-404 ZAP-70 Protein, active, 20 µg

Active, recombinant, human, full length ZAP-70 containing a C-terminal His6 tag, for use in Kinase Assays.

ZAP-70 Protein, active, 20 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
14-404
20 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-404
Brand Family Upstate
Trade Name
  • Upstate
DescriptionZAP-70 Protein, active, 20 µg
OverviewRecombinant, human, full length ZAP-70 containing a C-terminal His6 tag
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant, human, full length ZAP-70 containing a C-terminal His6 tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in Sf21
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Gene Symbol
  • ZAP70
  • ZAP-70
  • STD
  • TZK
  • SRK
Protein TargetZAP-70
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P43403 # Associates with the T-cell antigen receptor zeta chain (CD3Z). Plays a role in lymphocyte activation.
SIZE: 619 amino acids; 69872 Da
SUBUNIT: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB (By similarity). Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6 (By similarity).
TISSUE SPECIFICITY: Expressed in T- and natural killer cells.
DOMAIN: SwissProt: P43403 The SH2 domain binds to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z.
PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.
DISEASE:SwissProt: P43403 # Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily. & Contains 1 protein kinase domain. & Contains 2 SH2 domains.
Molecular Weight70 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1) substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -20C
Packaging Information
Material Size20 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-404 04053252581793

Documentation

ZAP-70 Protein, active, 20 µg SDS

Title

Safety Data Sheet (SDS) 

ZAP-70 Protein, active, 20 µg Certificates of Analysis

TitleLot Number
ZAP-70, active - 25510U-B 25510U-B
ZAP-70, active - D9CN010U-A D9CN010U-A

References

Reference overviewPub Med ID
The Syk family of protein tyrosine kinases in T-cell activation and development.
Chu, D H, et al.
Immunol. Rev., 165: 167-80 (1998)  1998

9850860 9850860

FAQ

QuestionAnswer
Some of my enzyme preparations require Brij-35, what is its purpose?The role of Brij-35 is to function as a mild detergent, which prevents aggregation in active kinases.

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-404M ZAP-70 Protein, active, 250 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases