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  • Millicoat CELL ADHESION STRIPS HUMAN LAMININ -

    Document Type:
    Certificate of Analysis
    Product Catalog Number:
    ECM103
    Product Catalog Name:
    Millicoat™ Human Laminin Coated Strips (96-Wells) (Millicoat™ Human Laminin Coated Strips (96-Wells))

  • Calreticulin has opposing effects on the migration of human trophoblast and myometrial endothelial cells. 22377355

    Calreticulin is a calcium binding, endoplasmic reticulum resident protein best known for its roles in intracellular calcium homeostasis and the quality control processes of the endoplasmic reticulum. There is evidence for a range of activities for calreticulin outside the endoplasmic reticulum, including in the cytosol, on the surface of different cells types and in the extracellular matrix. Recent evidence indicates that human pregnancy is a condition of elevated circulating calreticulin. Calreticulin was increased in the plasma of women throughout pregnancy compared to the non-pregnant state. Calreticulin was also further increased during the hypertensive disorder of human pregnancy, pre-eclampsia. To clarify the roles of circulating calreticulin in pregnancy and pre-eclampsia, the aim of this study was to determine the effects of exogenous calreticulin on two cell types that are relevant to normal human pregnancy and to pre-eclampsia. Human primary myometrial microvascular endothelial cells (UtMVEC-Myo) and the human trophoblast cell line, HTR8/SVneo, were cultured with exogenous calreticulin at concentrations (2 μg/ml and 5 μg/ml) comparable to that measured in maternal blood. The higher concentration of calreticulin significantly increased the migration of the UtMVEC-Myo cells, but significantly reduced the migration of the HTR8/SVneo cells. In the presence of only FGF, FBS and antibiotics calreticulin at 5 μg/ml significantly reduced the number of UtMVEC-Myo cells during in vitro culture for 120 h. These results demonstrate that exogenous calreticulin can alter both HTR8/SVneo and UtMVEC-Myo cell functions in vitro at a (patho-) physiologically relevant concentration. Increased calreticulin may also contribute to altered functions of both cell types during pre-eclampsia.
    Document Type:
    Reference
    Product Catalog Number:
    ECM205
    Product Catalog Name:
    Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105 (Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105)

  • Regulation of expression and role of leukemia inhibitory factor and interleukin-6 in the uterus of early pregnant pigs. 22920593

    Cytokines, which are generally involved in the process of inflammation, may also play a critical role in conceptus implantation. We examined: (1) the expression profiles of leukemia inhibitory factor (LIF) and interleukin (IL)-6 mRNA and their protein content in the endometrium of cyclic and pregnant gilts on Days 10 to 18 after estrus; (2) the effect of conceptus-exposed medium on LIF and IL-6 synthesis in the endometrium; (3) the profiles of IL6R and LIFR mRNA expression in pig conceptuses collected on Days 10 to 18 of pregnancy; and (4) the effect of LIF and IL-6 on the attachment and proliferation of porcine trophoblast cells. The expression of LIF mRNA in the endometrium increased between Days 10 and 12 in both cyclic and pregnant gilts, and tended to be higher in Day 12 pregnant animals compared with nonpregnant ones. The LIF protein content in the uterine lumen peaked on Day 12 of pregnancy, and was higher than on Day 12 of the estrous cycle. Endometrial IL-6 mRNA expression was upregulated on Day 12 in pregnant gilts compared with nonpregnant animals. Moreover, a higher content of IL-6 protein was observed in pregnant than in cyclic gilts. The addition of conceptus-exposed medium resulted in up-regulation of LIF and IL6 mRNA expression, and increased IL-6 content in endometrial slices. In conceptuses, increased mRNA expression was detected on Days 10 to 14 for IL6R and on Day 14 for LIFR, when compared with other days studied. LIF and IL-6 stimulated the attachment and proliferation of trophoblast cells in vitro. In summary, LIF and IL-6 are important components of embryo-uterine interactions during early pregnancy in the pig, and may contribute to successful conceptus implantation.
    Document Type:
    Reference
    Product Catalog Number:
    ECM101
    Product Catalog Name:
    Millicoat™ Human Fibronectin Coated Strips (96-Wells) (Millicoat™ Human Fibronectin Coated Strips (96-Wells))

  • MillicoatTM CELL ADHESION STRIPS HUMAN LAMININ

    Document Type:
    Certificate of Analysis
    Lot Number:
    2843123
    Product Catalog Number:
    ECM103
    Product Catalog Name:
    Millicoat™ Human Laminin Coated Strips (96-Wells) (Millicoat™ Human Laminin Coated Strips (96-Wells))

  • MillicoatTM CELL ADHESION STRIPS HUMAN LAMININ

    Document Type:
    Certificate of Analysis
    Lot Number:
    2944609
    Product Catalog Number:
    ECM103
    Product Catalog Name:
    Millicoat™ Human Laminin Coated Strips (96-Wells) (Millicoat™ Human Laminin Coated Strips (96-Wells))

  • MillicoatTM SCREENING KIT - 3501896

    Document Type:
    Certificate of Analysis
    Lot Number:
    3501896
    Product Catalog Number:
    ECM205
    Product Catalog Name:
    Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105 (Millicoat™ ECM Screening Kit, 1 ea. ECM101-ECM105)