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419700 Isopeptidase T, Rabbit

419700
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419700-25UGCN
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      塑膠安瓿;塑膠針藥瓶 25 μg
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      Description
      OverviewNative, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydrolases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity. Recommended reaction buffer: 25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 minutes with 10 mM DTT to achieve maximum activity.
      Catalogue Number419700
      Brand Family Calbiochem®
      SynonymsIpase-T
      References
      ReferencesDang, L.C., et al. 1998. Biochemistry 37, 1868.
      Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
      Stein, R.L., et al. 1995. Biochemistry 34, 12616.
      Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityFully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075)
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Isopeptidase T, Rabbit MSDS

      Title

      Safety Data Sheet (SDS) 

      Isopeptidase T, Rabbit Certificates of Analysis

      TitleLot Number
      419700

      References

      Reference overview
      Dang, L.C., et al. 1998. Biochemistry 37, 1868.
      Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
      Stein, R.L., et al. 1995. Biochemistry 34, 12616.
      Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Proteasomes Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2008 RFH
      SynonymsIpase-T
      DescriptionNative, rabbit isopeptidase T. A member of the higher molecular weight group of ubiquitin C-terminal hydroxylases (UCHs) involved in the hydrolysis of isopeptide linkages of polyUb chains. Ipase-T activity is regulated by ubiquitin levels. Plays a major role in ubiquitin recycling and in the regulation of 26S proteasome activity.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM HEPES, 1 mM DTT, pH 7.6.
      Recommended reaction conditions25 mM HEPES, 10 mM DTT, 25°C, pH 7.5. Pre-incubate the enzyme for 15 min with 10 mM DTT to achieve maximum activity.
      Purity≥95% by SDS-PAGE
      Biological activityFully functional as measured using 5 nM Ipase-T and 1 µM Ub-AMC (Cat. No. 662075)
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesDang, L.C., et al. 1998. Biochemistry 37, 1868.
      Melandri, F.D., et al. 1996. Biochemistry 35, 12893.
      Stein, R.L., et al. 1995. Biochemistry 34, 12616.
      Pickart, C.M., and Rose, I.A. 1986. J. Biol. Chem. 261, 10210.