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539638 PPI2, Human, Recombinant, E. coli - Calbiochem

539638
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539638-100UGCN
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      塑膠安瓿;塑膠針藥瓶 100 μg
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      Description
      OverviewRecombinant, human PPI2 expressed in E. coli. Inhibitor-2 is the regulatory subunit of the cytosolic type 1 Ser/Thr protein phosphatase-1 (PP1). It potently inhibits the activity of the free catalytic subunit of PP1 (IC50 = 1 nM).
      M.W. 30,000 (by SDS-PAGE).
      Catalogue Number539638
      Brand Family Calbiochem®
      SynonymsProtein Phosphatase Inhibitor 2
      References
      ReferencesYang, J., et al. 2000. J. Biol. Chem. 275, 22635.
      Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870.
      Helps, N.R., et al. 1994. FEBS Lett. 340, 93.
      Park, I., et al. 1994. J. Biol. Chem. 269, 944.
      Cohen, P. 1991. Methods Enzymol. 201, 389.
      Product Information
      ATP CompetitiveN
      FormLyophilized solid
      Hygroscopic Hygroscopic
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetactivity of free catalytic subunit of PP1
      Primary Target IC<sub>50</sub>1 nM inhibiting the activity of the free catalytic subunit of PP1
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      PPI2, Human, Recombinant, E. coli - Calbiochem MSDS

      Title

      Safety Data Sheet (SDS) 

      PPI2, Human, Recombinant, E. coli - Calbiochem Certificates of Analysis

      TitleLot Number
      539638

      References

      Reference overview
      Yang, J., et al. 2000. J. Biol. Chem. 275, 22635.
      Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870.
      Helps, N.R., et al. 1994. FEBS Lett. 340, 93.
      Park, I., et al. 1994. J. Biol. Chem. 269, 944.
      Cohen, P. 1991. Methods Enzymol. 201, 389.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2008 RFH
      SynonymsProtein Phosphatase Inhibitor 2
      DescriptionRecombinant, human PPI2 expressed in E. coli. Inhibitor-2 is the heat-stable, regulatory subunit of the cytosolic type 1 Ser/Thr protein phosphatase-1 (PP1). Inhibits the activity of the free catalytic subunit of PP1 (IC50 = 1 nM). Inhibition is reported to involve a "masking region" on the inhibitor that lies in close proximity to the active site of PP1 and blocks the access to substrates. Interaction of inhibitor-2 with the catalytic subunit of PP1 leads first to a rapid inhibition of the enzyme activity followed by the formation of a stable inactive complex. The inactive complex can be reactivated through phosphorylation of inhibitor-2 at Thr72 by glycogen synthase kinase-3 (GSK-3).
      FormLyophilized solid
      Purity≥95% by SDS-PAGE
      SolubilityReconstitute with 50 mM Tris-HCl, pH 7.0.
      Storage -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesYang, J., et al. 2000. J. Biol. Chem. 275, 22635.
      Huang, H.B., et al. 1999. J. Biol. Chem. 274, 7870.
      Helps, N.R., et al. 1994. FEBS Lett. 340, 93.
      Park, I., et al. 1994. J. Biol. Chem. 269, 944.
      Cohen, P. 1991. Methods Enzymol. 201, 389.