U6382

Anti-UCP-1 antibody produced in rabbit

Name: Anti-UCP-1 antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Ucp1 Antibody, Ucp1 Antibody - Anti-UCP-1 antibody produced in rabbit, Anti-Uncoupling Protein 1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD01092514

Product Name

Anti-UCP-1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~32 kDa

species reactivity

mouse, human, rat

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of mouse brown adipose tissue.
western blot: 1:1,000 using mitochondrial extract of rat brown adipose tissue

UniProt accession no.

P25874

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

300

Gene Information

human ... UCP1(7350)
mouse ... Ucp1(22227)
rat ... Ucp1(24860)

Application

Anti-UCP-1 antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Anti-UCP-1 specifically recognizes human, mouse and rat UCP-1. Staining of the UCP-1 band is specifically inhibited with the immunizing peptide. The epitopes recognized are compatible with routine formalin-fixation and paraffin-embedding.

Uncoupling proteins (UCPs) are transporters functioning as enzymatic uncouplers of oxidative phosphorylation. They can return protons pumped by the respiratory chain to the mitochondrial matrix. UCP-1 is exclusively expressed in brown adipose tissue (BAT) in rodents and in neonates where it is regulated by norepinephrine and thyroid hormones. UCP-1 has been shown to depend on CoQ (ubiquinone) as an obligatory co-factor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Uncoupling protein (UCP1) is a 32 kDa, mitochondrial inner membrane protein. It is active as a proton channel forming dimer. It can bind purine nucleotides and is capable of being stimulated by fatty acids.

Immunogen

synthetic peptide corresponding to human UCP1 sequence (amino acids 145-159) with N-terminal cysteine added, conjugated to KLH. The corresponding sequence is identical in rat and mouse UCP1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number

0000488169

0000475647

0000462568

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The molecular features of uncoupling protein 1 support a conventional mitochondrial carrier-like mechanism

Crichton PG, et al.

Biochimie, 134(12), 35-35 (2017)

The chemical uncoupler 2, 4-dinitrophenol (DNP) protects against diet-induced obesity and improves energy homeostasis in mice at thermoneutrality

Goldgof M, et al.

The Journal of Biological Chemistry, 289(28), 19341-19350 (2014)

Towards a molecular understanding of adaptive thermogenesis

Lowell BB, et al.

Nature, 404(6778), 652-660 (2000)

Brown adipose tissue improves whole-body glucose homeostasis and insulin sensitivity in humans.

Maria Chondronikola et al.

Diabetes, 63(12), 4089-4099 (2014-07-25)

Brown adipose tissue (BAT) has attracted scientific interest as an antidiabetic tissue owing to its ability to dissipate energy as heat. Despite a plethora of data concerning the role of BAT in glucose metabolism in rodents, the role of BAT

Severe burn injury induces thermogenically functional mitochondria in murine white adipose tissue

Porter C, et al.

Shock, 44(3), 258-258 (2015)

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