Anti-CD94 Antibody, clone 18d3

Natural killer cells antigen CD94 (UniProt: O54707; also known as Killer cell lectin-like receptor subfamily D member 1) is encoded by the Klrd1 (also known as Cd94) gene (Gene ID: 16643) in murine species. Natural killer (NK) cell antigen CD94 is a member of the C-type lectin superfamily and functions as part of a receptor complex involved in immune surveillance and regulation. Structurally, CD94 forms heterodimeric complexes with members of the NKG2 family, including NKG2A, NKG2C, and NKG2E. These complexes can exhibit inhibitory or activating functions, depending on the specific NKG2 isoform. This interaction allows NK cells to monitor global MHC class I expression levels, ensuring proper self/non-self discrimination and immune tolerance. CD94 is predominantly expressed on the surface of NK cells and certain T cell subsets. Its subcellular localization is restricted to the plasma membrane, where it interacts with adapter proteins such as DAP12/TYROBP, which are essential for its cell surface expression and signal transduction. CD94-NKG2A, the inhibitory receptor variant, transmits inhibitory signals upon binding to HLA-E, suppressing NK cell cytotoxic activity and cytokine production. Conversely, activating variants like CD94-NKG2C promote NK cell activity upon engagement with HLA-E, facilitating immune responses against infected or transformed cells. This dual functionality of CD94 plays a pivotal role in balancing immune activation and inhibition. Dysregulation of CD94 expression or function has been implicated in various pathological conditions such as rheumatoid arthritis, where synovial NK cells exhibit an activated phenotype regulated by this receptor complex. Additionally, CD94 has been studied in the context of cancer, where its interactions with HLA-E can influence immune escape mechanisms.

Stably transfected Chinese hamster Ovary (CHO) cells expressing the B6 alleles of CD94.

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 mouse splenocytes.

Flow Cytometry Analysis : 1.0 µg from a representative lot detected CD94 in one million C57BL/6 mouse splenocytes.

Flow Cytometry Analysis: A representative lot detected CD94 in Flow Cytometry application (Vance, R.E., et al. (1999). J Exp Med. 190(12):1801-12; Vance, R.E., et al. (2002). Proc Natl Acad Sci USA. 99(2):868-73; Orr, M.T., et al. (2010). PLoS One. 5(12):e15184).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 18d3 is a rat monoclonal antibody that detects CD94. It targets an epitope within the extracellular domain.

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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