Simultaneous determination of 45 antibacterial compounds in mushrooms - Agaricus bisporus by ultra-high performance liquid chromatography-tandem mass spectrometry.

A multi-residue method was developed for the determination of 45 antibacterial compounds in mushrooms - Agaricus bisporus. The method is based on liquid-liquid extraction with acetonitrile with addition of trichloroacetic acid and filtration through sodium sulphate, followed by the identification and quantification of the analyte residues by ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry (UHPLC-MS/MS). Satisfactory linearity was obtained for all compounds with regression coefficients greater than 0.99. The mean recoveries of antibacterial agents were in the range of 73% - 118%. Repeatability and intra-lab reproducibility were lower than 10% and 15%, respectively. The limit of detection ranged from 0.3-3.0 μg kg-1 and the limit of quantification was in the range of 1.0 - 10. 0 μg kg-1 depending on analyte. The proposed method can be successfully applied for the determination of antibiotics in commercially available mushrooms.