- Differential expression and anti-oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization.
Differential expression and anti-oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization.
FEBS letters (2014-03-19)
Xiu Xu, Jing-Yu Leng, Fei Gao, Zhen-Ao Zhao, Wen-Bo Deng, Xiao-Huan Liang, Yi-Juan Zhang, Zhi-Rong Zhang, Ming Li, Ai-Guo Sha, Zeng-Ming Yang
PMID24631040
ABSTRACT
Glutathione peroxidase 3 (GPX3) is an important member of antioxidant enzymes for reducing reactive oxygen species and maintaining the oxygen balance. Gpx3 mRNA is strongly expressed in decidual cells from days 5 to 8 of pregnancy. After pregnant mice are treated with GPX inhibitor for 3 days, pregnancy rate is significantly reduced. Progesterone stimulates Gpx3 expression through PR/HIF1α in mouse endometrial stromal cells. In the decidua, the high level of GPX3 expression is closely associated with the reduction of hydrogen peroxide (H2O2). Based on our data, GPX3 may play a major role in reducing H2O2 during decidualization.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Hydrogen peroxide solution, contains potassium stannate as inhibitor, 30-32 wt. % in water, semiconductor grade, 99.999% trace metals basis
Progesterone, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Hydrogen peroxide solution, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H2O
Sigma-Aldrich
Hydrogen peroxide solution, contains inhibitor, 30 wt. % in H2O, meets USP testing specifications
Supelco
Progesterone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®