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Merck

G6048

Galactokinase human

recombinant, expressed in E. coli

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:

Product Name

Galactokinase human, recombinant, expressed in E. coli

recombinant

expressed in E. coli

form

solution

specific activity

≥1400 unit/μg protein

mol wt

42 kDa

shipped in

dry ice

storage temp.

−70°C

Quality Level

Biochem/physiol Actions

Galactokinase catalyzes the phosphorylation of αD-galactose to produce galactose-1-phosphate as part of the Leloir pathway.

General description

N-terminal GST-tagged 42 kDa full length protein

Other Notes

One unit will convert 1.0 picomole of galactose to galactose-1-phosphate per minute at pH 7.4 at 30 °C.

Physical form

Supplied as a solution in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT and 10-250 mM imidazole.

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Dariusz Abramczyk et al.
Eukaryotic cell, 11(3), 334-342 (2012-01-03)
The regulation of the Saccharomyces cerevisiae GAL genes in response to galactose as a source of carbon has served as a paradigm for eukaryotic transcriptional control over the last 50 years. Three proteins--a transcriptional activator (Gal4p), an inhibitor (Gal80p), and
Shivani Malik et al.
Nucleic acids research, 40(8), 3348-3363 (2011-12-27)
Recently, we have demonstrated a predominant association of Rad26p with the coding sequences but not promoters of several GAL genes following transcriptional induction. Here, we show that the occupancy of histone H2A-H2B dimer at the coding sequences of these genes
David Drew et al.
Methods in molecular biology (Clifton, N.J.), 866, 41-46 (2012-03-29)
Expression plasmids for Saccharomyces cerevisiae offer a wide choice of vector copy number, promoters of varying strength and selection markers. These expression plasmids are usually shuttle vectors that can be propagated both in yeast and bacteria, making them useful in
Sarah Piccirillo et al.
BMC biotechnology, 11, 120-120 (2011-12-07)
Precise targeted mutations are defined as targeted mutations that do not require the retention of other genetic changes, such as marker genes, near the mutation site. In the yeast, S. cerevisiae, there are several methods for introducing precise targeted mutations
Bridget L Baumgartner et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(52), 21087-21092 (2011-12-14)
Cells have evolved complex regulatory networks that reorganize gene expression patterns in response to changing environmental conditions. These changes often involve redundant mechanisms that affect various levels of gene expression. Here, we examine the consequences of enhanced mRNA degradation in

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