Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Stem cells have the ability to generate every cell in the body. Elucidating this process will have a profound impact on our understanding of animal development and will help produce therapies to regenerate damaged and diseased tissues. Because cell differentiation often involves a dramatic metamorphosis of the stem cell into a committed cell lineage, Amnis® imaging flow cytometry is ideally suited for these types of studies. Whether by measuring the location of proteins that help stem cells maintain pluripotency or by quantifying signaling events that lead toward differentiation down a committed cell line, the ability of Amnis® imaging flow cytometry to analysis large numbers of cells simplifies identification and classification of rare stem cell populations.
Octamer binding proteins are believed to allow cycling stem cells to remain in a pluripotent state. Understanding how stem cells can proliferate without committing to a cell lineage can help produce stable cell lines of progenitor cells as well as describe the biology of differentiation. Here we quantify the expression pattern of Oct34 (orange) in CD9 (green) positive stem cells.
Erythroid Differentiation
As hematopoeitc stem cells differentiate through the erythroid lineage, they shrink and eventually enucleate as they progress towards becoming RBC. They also progressively gain expression of glycophorinA and lose CD71 expression. The extruded nuclei express low levels of glycophorinA on their membranes. This experiment demonstrates the unique ability of the Imagestream®x to classify cells using a combination of intensity, morphology, and location based parameters.
Stem cells have the ability to generate every cell in the body. Elucidating this process will have a profound impact on our understanding of animal development and will help produce therapies to regenerate damaged and diseased tissues. Because cell differentiation often involves a dramatic metamorphosis of the stem cell into a committed cell lineage, Amnis® imaging flow cytometry is ideally suited for these types of studies. Whether by measuring the location of proteins that help stem cells maintain pluripotency or by quantifying signaling events that lead toward differentiation down a committed cell line, the ability of Amnis® imaging flow cytometry to analysis large numbers of cells simplifies identification and classification of rare stem cell populations.
