1.52001
Chromolith® RP-18 endcapped (monolithic) HPLC Columns
L × I.D. 100 mm x 3 mm HPLC Column
Synonym(s):
Chromolith® Performance RP-18 endcapped 100-3 HPLC column
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About This Item
UNSPSC Code:
41115709
NACRES:
SB.52
Matrix active group:
C18 bonding phase
Pore size:
13 nm mesoporosity, 2 μm macroporosity
Matrix:
monolithic silica
Product Name
Chromolith® RP-18 endcapped, L × I.D. 100 mm x 3 mm HPLC column
material
PEEK column
Quality Level
agency
suitable for USP L1
product line
Chromolith®
feature
endcapped
packaging
1 ea of
extent of labeling
18% Carbon loading
parameter
200 bar pressure, 45 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
10 cm × 3 mm
matrix
monolithic silica
matrix active group
C18 bonding phase
pore size
13 nm mesoporosity, 2 μm macroporosity
operating pH range
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
General description
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® Performance RP-18 endcapped 100-3 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped 100-3 columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® Performance RP-18 endcapped 100-3 columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 3 mm I.D. is the best choice for fast separations of complex samples on any HPLC and UHPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-3 guard cartridges (3 pieces) [1.52005] with the Chromolith® Guard Cartridge Holder 5 mm x 3 mm [1.52004]
Holder and guard cartridges are also available as Guard cartridge set [1.52008]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® Performance RP-18 endcapped 100-3 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® Performance RP-18 endcapped 100-3 columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® Performance RP-18 endcapped 100-3 columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 3 mm I.D. is the best choice for fast separations of complex samples on any HPLC and UHPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-3 guard cartridges (3 pieces) [1.52005] with the Chromolith® Guard Cartridge Holder 5 mm x 3 mm [1.52004]
Holder and guard cartridges are also available as Guard cartridge set [1.52008]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Application
- Rapid Determination of 30 Polyphenols in Tongmai Formula: This study utilized the Chromolith® RP-18 endcapped column in a liquid chromatography-tandem mass spectrometry setup to analyze 30 polyphenolic compounds in a traditional Chinese medicine formula, demonstrating its application in pharmaceutical analysis and quality control of complex herbal formulations (Wang et al., 2017).
- Ultra-fast gradient LC method for omeprazole analysis using a monolithic column: The Chromolith® RP-18 endcapped column was employed in an ultra-fast gradient liquid chromatography method for the analysis of omeprazole in enteric-coated pellets, highlighting its efficiency in pharmaceutical quality control and rapid method development (Borges et al., 2010).
Analysis Note
Appearance: passes test
Pressure: ≤ 30 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.86 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.35 - 2.65
Pressure: ≤ 30 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.86 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.35 - 2.65
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
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