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Cytiva®

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A collection of amber glass bottles with white labels, containing cell separation media such as Ficoll-Paque<sup>®</sup> and Percoll<sup>®</sup>, against a yellow background. Several amber glass bottles of varying sizes are arranged in two rows. The bottles have white labels with black text.

Ficoll-Paque™ PLUS Density Gradient Media

An array of Cytiva™ bioprocess products displayed against a blue background. The collection includes several white plastic bottles with labels and red-capped syringes.

Glutathione Sepharose™ 4 Fast Flow Resin

A set of three bottles with colored caps in red, yellow, and blue. These bottles are placed in front of a box. Cy3® and Cy5® fluorescent labels are used in scientific research for tagging and visualizing molecules under specific lighting conditions.

Amersham® CyDye DIGE Fluor Kits

A product kit for nucleic acid preparation by Illustra™. Beside the box are three bottles. The entire setup is presented against a solid yellow background.

illustra™ Nucleic Acid Purification

Four boxes of Whatman laboratory supplies including Blotting Papers, Membranes, & FTA Cards against a green background. The products feature an orange and white design and descriptive text on their labels.

Hybond™ Blotting Paper

A collection of Cytiva labware products including filtration devices, bottles, syringes, and membrane discs, arranged neatly on a blue background.

Amersham® ECL Western Blotting Detection Reagent

Cytiva® is a leading global Life Science company that provides a comprehensive portfolio of tools and technologies designed to empower researchers and biopharma companies at every stage, from discovery to delivery. Renowned for setting the gold standard in chromatography, Cytiva® resins are integral to 75% of FDA-approved biotherapeutic manufacturing processes. Choose from trusted resins like MabSelect® and streamline your chromatography workflows with AKTA® systems that offer automation and efficiency. Additionally, explore the industry's most diverse portfolio of filtration solutions with Whatman® and Pall®. With Amersham®, you can take the guesswork out of western blotting. Join us in advancing science and improving lives—together, we can drive breakthroughs that make a meaningful difference in the world.

Products

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Chromatography

Researchers worldwide rely on AKTA® systems and Cytiva® prepacked columns and resins for the isolation and purification of target molecules. No matter the molecule or workflow, Cytiva® has a solution tailored for every step. Our offerings include:

  • Affinity Resins: Choose from trusted options like MabSelect®, Protein Select®, His-tag, GST-tag, Strep-tag, MBP tagged resins, Protein A, Protein G, and Protein L.
  • Size Exclusion Resins: Utilize our reliable Sepharcyl®, Superose®, and Superdex® resins.
  • Ion Exchange Techniques: Leverage Capto® and SOURCE® for efficient separation.
  • Hydrophobic Interaction Resins: Explore options such as Octyl, Phenyl, and Butyl.

Filtration

Cytiva® proudly offers the industry's most comprehensive selection of filtration devices and membranes through Whatman® and Pall®.

  • Whatman® Solutions: Backed by 250 years of expertise, Whatman® products are designed to work effectively, every time. Choose from a variety of membranes, including cellulose, glass, quartz, nylon, PVDF, PTFE, and PES. Explore our filtration devices like Uniflo™ and Puradisc™ syringe filters, as well as Uniprep™ syringeless filters.
  • Pall® Solutions: With Pall® now part of our portfolio, you gain access to both foundational and cutting-edge Life Science filtration solutions. The Acrodisc® syringe filter is a trusted choice, and you can select from high-quality membranes such as Supor®, Nylaflo®, Versapor®, Metricel®, Emfab®, TissuQuartz™, PVC, PTFE, and glass. Additionally, rely on the extensive Pall® filter funnel portfolio for your microbiology and testing needs, and concentrate your samples with Nanosep®, Microsep®, Macrosep®, and Jumbosep® centrifugal filters.

Western Blotting

Amersham®, the pioneer of enhanced chemiluminescence (ECL) detection, continues to innovate its portfolio to meet all your western blotting needs. Choose ECL Prime for the most sensitive ECL system available. Utilize DualVue™ and Rainbow markers to ensure accurate and precise results. Amersham® also offers high-quality nitrocellulose and PVDF blotting membranes with pore sizes ranging from 0.1 to 0.45 µm. Finally, employ Pharmalyte® carrier ampholytes for isoelectric focusing across a pH gradient, allowing for the effective separation of charged molecules (proteins or peptides) based on their isoelectric point (pI).

Gradient Density Media

Ficoll® and Percoll® are globally recognized standards for mediums designed to isolate mononuclear cells, viruses, and subcellular particles through gradient density centrifugation.

Magnetic Beads

Elevate your assays and purification methods with Sera-Mag® SpeedBeads. These amine-blocked magnetic particles are uniform, colloidally stable, mono-dispersed, and non-porous super-paramagnetic spheres, produced using a proprietary core-shell method. The core consists of a carboxylate-modified particle made through free radical emulsion polymerization of styrene and acid monomer. Magnetite (Fe3O4) is coated onto this core particle and encapsulated with proprietary polymers, while the final surface is treated with a specialized method to minimize nonspecific protein binding.

Genomics

Cytiva® offers a rapid suite of tools for working with nucleic acids. Use Illustra® kits for extraction, along with NAP®, Sephadex®, and RNAspin columns for nucleic acid sample cleanup. Discover rolling circle amplification with Phi29, which provides higher fidelity amplification compared to traditional PCR methods.

Related Resources

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Related Product Categories
Laboratory Sample Collection
Protect and manage your samples with solutions including vials, tubes, bottles, dishes, plates, boxes, and racks for collection, transport, tracing, and storage needs.
Syringe Filters
High performance sterile and nonsterile syringe filters offered in a variety of membrane materials and pore sizes, tailored for sample preparation for HPLC, UHPLC, ion chromatography, dissolution testing, and other analyses.
Filter Paper
Whatman® filter papers for qualitative and quantitative analysis.
Filter Membranes
Enhance HPLC and analysis with membranes filtering solvents, aqueous solutions. Ideal for prep, clarity, removal, sterilization, separation.
Recombinant/Fusion Tag Protein Purification
Optimize protein purification with our range of affinity resins and buffers for multiple tags and purification techniques.
Antibody Protein Purification
Antibody purification resins and prepacked columns for lab scale, process scale, and industry scale workflows.
Biotinylated Protein Purification
Streptavidin and avidin resins and prepacked columns for small- to large-scale purification of biotinylated proteins and antibodies.
Cell Culture Supplements & Reagents
Browse high-quality cell culture supplements, including sera, growth factors, cytokines, hormones, and freezing reagents.
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Cell Therapy Manufacturing
Introducing a new cell therapy demands optimal performance in upstream and downstream processing, process development, testing, and manufacturing. World-class products and partners are critical to success, speeding advancement of therapeutics that are effective, safe, and affordable.
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Monoclonal antibody manufacturing requires scalable solutions ensuring high therapeutic concentration and process safety for immunotherapy.
Live Cell Imaging Analysis
Live cell imaging tools enable analysis in cell trafficking, gene expression, migration, 3D culture, and cytoskeletal dynamics.
Lysis & Protein Extraction
An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.
Gel Electrophoresis
Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.
Sample Preparation by Filtration
Filtration separates substances to reduce sample complexity and increase analyte purity, improving analytical results.
Protein Purification
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Western Blotting
An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
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