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Merck

CE0050

CelLytic MEM Protein Extraction Kit

Sinónimos:

Membrane protein extraction kit

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NACRES:
NA.56
UNSPSC Code:
41116134
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technique(s)

protein extraction: suitable

Quality Segment

shipped in

wet ice

storage temp.

−20°C

General description

CelLytic MEM Protein Extraction Kit offers a fast and convenient method to isolate hydrophobic and raft microdomain associated proteins from cells. The method is based on phase separation and does not require cell membrane isolation. The separated proteins can be used for further experiments such as SDS-PAGE, Western blotting, dot blotting, and immunoprecipitation. The kit has been tested on, but not limited to, HeLa, HEK-293, NIH 3T3, COS and CHO cell lines.
Membrane proteins make up around 20-30% of an organism′s genome and serve as cellular gatekeepers, regulators, and sensors. They have diverse cellular functions, such as shielding the cell from external toxins, being the starting point of intracellular signaling cascades, and retaining critical ion concentrations.

Application

CelLytic MEM Protein Extraction Kit has been used for de novo lipogenesis measurements using hepatocytes and to extract membrane proteins for western blotting.
Sufficient reagents supplied for 80 tests.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC


Solo componentes del kit

Referencia del producto
Descripción

  • Lysis and Separation Buffer 50 mL

  • Wash Buffer for CelLytic MEM 50 mL

  • Sodium Chloride, 4M Solution 1.5 mL

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS & Precios

  • Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 1 mL
    SDS

pictograms

Exclamation mark

Clase de almacenamiento

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

wgk

WGK 2

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2



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