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374089 Anti-Heme Oxygenase-1 (1-261) Rabbit pAb

374089
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~32 kDa HO-1. Does not cross-react with HO-2.
Catalogue Number374089
Brand Family Calbiochem®
SynonymsAnti-HO-1, Anti-Hsp32
Application Data
Detection of heme oxygenase-1 by immunoblotting. Samples: Purified SPP-730 (lane 1), extracts from human liver microsomes (lane 2), rat liver microsomes (lane 3), mouse liver microsomes (lane 4), and canine liver microsomes (lane 5). Primary antibody: Anti-Heme Oxygenase-1 (1-261) Rabbit pAb (Cat. No. 374089) (1:1000). Detection: chemiluminescence.

Detection of human heme oxygenase-1 by immunohistochemistry. Sample: Alzheimer's disease brain tissue. Primary antibody: Anti-Heme Oxygenase-1 (1-261) Rabbit pAb (Cat. No. 374089) (assay dependent). Detection:
References
ReferencesSmith, M., et al. 1994. Am. J. Path. 145, 42.
Shibahara, S., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7865.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol.
Positive controlRecombinant rat HO-1 (Hsp32) protein or recombinant human HO-1 (Hsp32) protein
Preservative≤0.1% sodium azide
Applications
Key Applications Flow Cytometry
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunohistochemistry
Immunoprecipitation
Application NotesFlow Cytometry (see comments)
Immunoblotting (1:500, colorimetric; 1:2000, chemiluminescence)
Immunohistochemistry (see comments)
Immunoprecipitation (1:100)
Immunocytochemistry (see comments)
Application CommentsMay cross-react with HO-2 in immunoblotting. The signal is weaker in total cell lysates due to the low abundance of HO-1. Additionally, the antibody recognizes a ~50 kDa band present only in transformed cell lines, not in normal tissues. This antibody has also been reported to work in immunohistochemistry, immunoelectron microscopy, and flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenrecombinant rat HO-1 consisting amino acids 1-261 (lacks the membrane spacing region)
ImmunogenRat
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C or -70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
374089 0

Documentation

Anti-Heme Oxygenase-1 (1-261) Rabbit pAb Certificates of Analysis

TitleLot Number
374089

References

Reference overview
Smith, M., et al. 1994. Am. J. Path. 145, 42.
Shibahara, S., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7865.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-March-2008 JSW
SynonymsAnti-HO-1, Anti-Hsp32
ApplicationFlow Cytometry (see comments)
Immunoblotting (1:500, colorimetric; 1:2000, chemiluminescence)
Immunohistochemistry (see comments)
Immunoprecipitation (1:100)
Immunocytochemistry (see comments)
Application Data
Detection of heme oxygenase-1 by immunoblotting. Samples: Purified SPP-730 (lane 1), extracts from human liver microsomes (lane 2), rat liver microsomes (lane 3), mouse liver microsomes (lane 4), and canine liver microsomes (lane 5). Primary antibody: Anti-Heme Oxygenase-1 (1-261) Rabbit pAb (Cat. No. 374089) (1:1000). Detection: chemiluminescence.

Detection of human heme oxygenase-1 by immunohistochemistry. Sample: Alzheimer's disease brain tissue. Primary antibody: Anti-Heme Oxygenase-1 (1-261) Rabbit pAb (Cat. No. 374089) (assay dependent). Detection:
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~32 kDa HO-1 protein.
BackgroundHeme oxygenase (HO) is a microsomal enzyme that oxidatively cleaves heme, a pro-oxidant, into carbon monoxide and biliverdin, which is reduced to the antioxidant, bilirubin. Two isozymes, termed HO-1 and HO-2, have been identified. By SDS-PAGE, mammalian HO-1 is ~31-33 kDa; HO-2 is ~36 kDa. HO-1, also referred to as stress protein Hsp32, is the inducible form of the enzyme. HO-1 expression can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO-2 is constitutively expressed. HO-2 is the most prevalent form in most tissues, except the spleen where HO-1 levels are predominant.
HostRabbit
Immunogen speciesRat
Immunogenrecombinant rat HO-1 consisting amino acids 1-261 (lacks the membrane spacing region)
IsotypeIgG
Speciescanine, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep
Positive controlRecombinant rat HO-1 (Hsp32) protein or recombinant human HO-1 (Hsp32) protein
FormLiquid
FormulationIn PBS, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsMay cross-react with HO-2 in immunoblotting. The signal is weaker in total cell lysates due to the low abundance of HO-1. Additionally, the antibody recognizes a ~50 kDa band present only in transformed cell lines, not in normal tissues. This antibody has also been reported to work in immunohistochemistry, immunoelectron microscopy, and flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C or -70°C).
Toxicity Standard Handling
ReferencesSmith, M., et al. 1994. Am. J. Path. 145, 42.
Shibahara, S., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7865.