Enterococci 100

Enterococci 100 medium is designed for the selective detection of enterococci and D-streptococci, which are key indicators of fecal contamination. The medium contains sodium azide, which suppresses the growth of many accompanying microorganisms, particularly Gram-negative bacteria, while allowing enterococci to grow. It also includes the chromogenic substrate X-GLU (5-bromo-4-chloro-3-indolyl-ß-D-glucopyranoside). This substrate is cleaved by ß-D-glucosidase, an enzyme characteristic of enterococci, resulting in an intense blue-green color. This color change provides a clear visual indication of the presence of enterococci and D-streptococci.

Enterococci 100 medium is used for the detection and confirmation of enterococci and D-streptococci in water samples. It is particularly used in food and beverage testing for assessing fecal contamination in drinking water, process water, environmental water, and other water sources where microbiological quality must be monitored. The selective and chromogenic properties of the medium support rapid, easy-to-read results and help reduce false positives from other ß-D-glucosidase-positive bacteria.

Appearance
- clearness:
clear
- colour: yellowish to yellowish-brown
pH-value (25 °C): 7.3 - 7.7
Inoculum on reference medium
- Enterococcus faecalis ATCC 19433 (WDCM 00009):
10 - 100
- Enterococcus durans ATCC 19432: 10 - 100
- Staphylococcus aureus ATCC 25923 (WDCM 00034): ≥ 1000
Growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009):
fair to good
- Enterococcus durans ATCC 19432: fair to good
- Staphylococcus aureus ATCC 25923 (WDCM 00034): fair to good
Colour change to green-blue
- Enterococcus faecalis ATCC 19433 (WDCM 00009):
+
- Enterococcus durans ATCC 19432: +
- Staphylococcus aureus ATCC 25923 (WDCM 00034): -
Incubation: 24 - 48 hrs.; 35 °C; aerobic.

Replaces: 1.01299.0002

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