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1.07228

Buffered Peptone Water

GranuCult® prime, ISO 19250, ISO 6579-1, ISO 21528-1, ISO 22964, FDA-BAM, EP, granular, pkg of 0.5-25 kg

Synonym(s):

BPW, Buffered Peptone Solution, GranuCult® BPW, Peptone Water (buffered), Peptone Water, buffered, Peptone Water, phosphate-buffered, Tryptone Phosphate Water

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106213
Manufacturer/tradename:
GranuCult® prime
Agency:
APHA, EP, FDA-BAM, ISO 19250, ISO 21528-1, ISO 22964, ISO 6579-1, ISO 6887
Ph:
7.2 (37 °C, 25 g/L in H2O, after autoclaving)
Form:
granular
Suitability:
Cronobacter spp., Salmonella spp., enterobacteriaceae, nonselective for bacteria (preliminary enrichment)
Application(s):
food and beverages
microbiology
pathogen testing
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Quality Level

200

agency

APHA, EP, FDA-BAM, ISO 19250, ISO 21528-1, ISO 22964, ISO 6579-1, ISO 6887

sterility

non-sterile

form

granular

packaging

pkg of 0.5-25 kg

manufacturer/tradename

GranuCult® prime

technique(s)

microbiological culture: suitable

pH

7.2 (37 °C, 25 g/L in H2O, after autoclaving)

solubility

25.5 g/L

bulk density

800 kg/m3

application(s)

food and beverages
microbiology
pathogen testing

storage temp.

15-25°C

suitability

Cronobacter spp., Salmonella spp., enterobacteriaceae, nonselective for bacteria (preliminary enrichment)

General description

Buffered Peptone Water is rich in nutrients and produces high resuscitation rates for sub lethally injured bacteria and intense growth. The phosphate buffer system prevents bacterial damage caused by changes in the pH of the medium. Peptone including enzymatic digest of casein acts as a source of carbon, nitrogen, vitamins and minerals whilst sodium chloride maintains the osmotic balance.
GranuCult® is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Application

Buffered Peptone Water is used in the testing of food and water samples. This product is suitable for the preliminary non selective enrichment of bacteria, particularly pathogenic Enterobacteriaceae such as Salmonella and Cronobacter, from food, animal feed, water, and other materials. The product is also suitable as a diluent for general use.

Preparation Note

  • Store at +15 °C to +25 °C, dry and tightly closed.
  • Do not use clumped or discolored medium.
  • Protect from UV light (including sunlight).
  • For in vitro use only.
  • According to EN ISO/FDIS 6579-1, the self-prepared medium can be stored in closed containers at +2 °C to +8 °C in the dark and protected against evaporation for up to six months.

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish
pH-value (25 °C): 6.8 - 7.2
Growth promotion test in accordance with the current version of DIN EN ISO 11133 and the harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≤ 100
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Salmonella Abony NCTC 6017): ≤ 100
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Salmonella enteritidis ATCC 13076 (WDCM 00030)): good to very good
Growth (Salmonella Abony NCTC 6017): good to very good
Incubation:
18 ± 2 hours at 37 ± 1 °C
S.abony 18 - 24 hours at 30 - 35 °C

Other Notes

Replaces: 1072285007; 1.07228
The prepared medium is clear and yellowish.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture medi. depending on your needs. The designations basi. plu. or prime are added to indicate the quality control leve. from basic quality control to standard QC plus to prime for full regulatory compliance.

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Storage Class

11 - Combustible Solids

wgk

WGK 1

Certificates of Analysis (COA)

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